Mann J R, Gadi I, Harbison M L, Abbondanzo S J, Stewart C L
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Cell. 1990 Jul 27;62(2):251-60. doi: 10.1016/0092-8674(90)90363-j.
The inviability of diploid androgenetic and parthenogenetic embryos suggests imprinting of paternal and maternal genes during germ cell development, and differential expression of loci depending on parental inheritance appears to be involved. To facilitate identification of imprinted genes, we have derived diploid androgenetic embryonic stem (ES) cell lines. In contrast to normal ES cells, they form tumors composed almost entirely of striated muscle when injected subcutaneously into adult mice. They also form chimeras following blastocyst injection, although many chimeras die at early postnatal stages. Surviving chimeras develop skeletal abnormalities, particularly in the rib cartilage. These results demonstrate that androgenetic ES cells are pluripotent and point to stage- and cell-specific expression of developmentally important imprinted genes.
二倍体孤雄生殖和孤雌生殖胚胎的 inviability 表明生殖细胞发育过程中父本和母本基因的印记，并且似乎涉及取决于亲本遗传的基因座差异表达。为便于鉴定印记基因，我们已获得二倍体孤雄生殖胚胎干细胞（ES）系。与正常 ES 细胞相比，将它们皮下注射到成年小鼠体内时会形成几乎完全由横纹肌组成的肿瘤。在囊胚注射后它们也会形成嵌合体，尽管许多嵌合体在出生后早期死亡。存活的嵌合体出现骨骼异常，尤其是肋软骨。这些结果表明孤雄生殖 ES 细胞具有多能性，并指出发育重要的印记基因的阶段和细胞特异性表达。
