Department of Biomedical Engineering, Texas A and M University, College Station, Texas - USA; Orthopeutics, L.P., Lexington, KY - USA.
J Appl Biomater Funct Mater. 2013 Sep 13;11(2):e73-9. doi: 10.5301/JABFM.5000151.
Quantify changes in the flexion--extension neutral zone of the intervertebral disc with injections of increasing genipin concentration.
Bovine motion segments were treated with varying concentrations of genipin using bilateral injections of constant volume. After overnight static compression loading of the treated segments, anterior-posterior offset loading was used to simulate flexion-extension motion. Range of motion, neutral zone length, neutral zone stiffness, and an instability score were measured.
Injection of the disc annulus with increasing concentrations of genipin resulted in corresponding changes in flexion-extension neutral zone. A minimum concentration of 40 mM was needed to observe a significant change. The largest changes were observed with the 400 mM injection. Netural zone stability was the most sensitive of the metrics with a percent change of 48% at 40 mM and over 200% at 400 mM.
This study establishes the efficacy of using injection delivery to affect disc joint mechanics and quantifies the dose response between injected genipin and the flexion-extension stability of the disc.
定量研究随着注射用京尼平浓度的增加，椎间盘屈伸中性区的变化。
采用双侧等容量注射的方法，用不同浓度的京尼平处理牛运动节段。在对处理节段进行过夜静态压缩加载后，采用前后偏移加载模拟屈伸运动。测量运动范围、中性区长度、中性区刚度和不稳定性评分。
向椎间盘环注射浓度逐渐增加的京尼平，导致屈伸中性区发生相应变化。需要至少 40mM 的浓度才能观察到显著变化。400mM 注射观察到的变化最大。在所有指标中，中性区稳定性最敏感，在 40mM 时的百分比变化为 48%，在 400mM 时超过 200%。
本研究确立了使用注射输送来影响椎间盘关节力学的有效性，并定量研究了注射用京尼平和椎间盘屈伸稳定性之间的剂量反应关系。
