开发一种新型的 FRET 探针，用于实时测定神经酰胺酶活性。
Development of a novel FRET probe for the real-time determination of ceramidase activity.
机构信息
Department of Organic & Bioorganic Chemistry, Humboldt Universität zu Berlin, Institute for Chemistry, Brook-Taylor Str-2, 12489 Berlin, Germany.
出版信息
Chembiochem. 2013 Jun 17;14(9):1049-52. doi: 10.1002/cbic.201300207. Epub 2013 May 31.
PMID:23728798
Abstract
Fretful novelty: We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. We present a fluorescent sphingolipid FRET probe that allows homogeneous ratiometric determination of enzyme activity in real-time.
摘要
烦躁的新奇
我们开发了两种新型的双标记荧光神经酰胺类似物，它们表现出显著的 FRET，并被神经酰胺酶水解。我们提出了一种荧光神经鞘脂 FRET 探针，允许实时进行同相比率测定酶活性。
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