Faculty of Public Health, Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry of Education, Key Laboratory of Trace Elements and Endemic Diseases of Ministry of Health, Xi'an 710061, China.
Sci China Life Sci. 2013 Sep;56(9):797-803. doi: 10.1007/s11427-013-4495-z. Epub 2013 Jun 1.
In this study, differentially expressed genes in peripheral blood from patients with Kashin-Beck disease and Keshan disease were compared to further investigate the etiology and pathogenesis of both diseases, which occur in a common endemic area of China. Twenty Kashin-Beck disease patients and 12 healthy controls, and 16 Keshan disease patients and 16 healthy controls, were grouped into four pairs. Patients and controls were selected from common endemic areas for the two diseases. Total RNA was isolated from peripheral blood mononuclear cells from all patients and controls, and gene expression profiles analyzed by oligonucleotide microarrays. Sixteen genes differentially expressed in both Kashin-Beck disease and Keshan disease (versus controls) were identified, and comprised nine genes showing synchronous and seven asynchronous expression. The Comparative Toxicogenomics Database shows that expression and biological function of these genes can be affected by multiple environmental factors, including mycotoxin and selenium content, potential environmental risk factors for the two diseases. Thus, these shared differentially expressed genes may contribute to the distinct organ lesions, caused by common environmental risk factors of Kashin-Beck disease and Keshan disease.
在这项研究中，比较了克山病和大骨节病患者外周血中的差异表达基因，以进一步探讨这两种疾病在中国共同流行地区的病因和发病机制。将 20 名大骨节病患者和 12 名健康对照者，以及 16 名克山病患者和 16 名健康对照者分为四组。患者和对照者均选自两种疾病的共同流行地区。从所有患者和对照者的外周血单核细胞中分离总 RNA，并通过寡核苷酸微阵列分析基因表达谱。鉴定出在大骨节病和克山病（与对照相比）中均差异表达的 16 个基因，其中包括 9 个同步表达和 7 个异步表达的基因。比较毒理学基因组学数据库显示，这些基因的表达和生物学功能可能受到多种环境因素的影响，包括真菌毒素和硒含量，这是两种疾病的潜在环境危险因素。因此，这些共同差异表达的基因可能导致大骨节病和克山病的共同环境危险因素引起的不同器官病变。
