印度导致番茄鞋带病的黄瓜花叶病毒分离株的生物学和分子特征，该分离株与黄瓜花叶病毒的欧洲或东亚分离株亲缘关系更近。
Biological and Molecular Characterization of Cucumber mosaic virus Isolate Causing Shoestring Disease of Tomato in India which has Closer Affinity to European or East Asian Isolates of CMV.
作者信息
Pratap D, Kumar S, Snehi S K, Raj S K
机构信息
Plant Molecular Virology, CSIR-National Botanical Research Institute, Lucknow, 226 001 India.
出版信息
Indian J Virol. 2012 Jun;23(1):57-63. doi: 10.1007/s13337-012-0059-2. Epub 2012 Mar 14.
An isolate of Cucumber mosaic virus (CMV) retrieved from tomato in India was characterized based on its transmission by sap inoculations, Western blot immuno-assay and sequence analysis of RNA3 and 2b genome. The RNA3 genome was of 2,220 nucleotides (nt) which contained two ORFs: movement protein of 852 nt translating 283 amino acids and coat protein of 657 nt translating 218 amino acids. The complete sequence of RNA3 geneome (Acc. EF153734) shared highest 98-99% identities with P1-1, Tfn, and Nt9 strains of CMV infecting tomato reported from abroad. The 333 nucleotides long RNA2b gene (Acc. EF710773) also showed highest 98% identities with P1-1 and 97% with Tfn and NT9 strains of CMV but only 83-84% identities with Indian strains of CMV. Therefore, the isolate under study was identified as a new isolate of CMV of subgroup IB based on highest sequence similarities and closer affinity to European or East Asian isolates of CMV.
从印度番茄中分离得到的一株黄瓜花叶病毒（CMV），通过汁液接种传播、蛋白质免疫印迹分析以及RNA3和2b基因组序列分析对其进行了特征鉴定。RNA3基因组由2220个核苷酸（nt）组成，包含两个开放阅读框：852 nt的移动蛋白翻译出283个氨基酸，657 nt的外壳蛋白翻译出218个氨基酸。RNA3基因组的完整序列（登录号EF153734）与国外报道的感染番茄的CMV的P1-1、Tfn和Nt9株系具有最高98%-99%的同一性。333个核苷酸长的RNA2b基因（登录号EF710773）与CMV的P1-1株系也具有最高98%的同一性，与Tfn和NT9株系具有97%的同一性，但与印度CMV株系只有83%-84%的同一性。因此，基于最高的序列相似性以及与欧洲或东亚CMV分离株更密切的亲缘关系，所研究的分离株被鉴定为CMV亚组IB的一个新分离株。
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