Pokorny K S, Kenyon K R, Swinger C, Barker B A, Henriquez A S, Barraquer J I, Amin D, Schmitterer M, Hanninen L A
Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07107.
Cornea. 1990 Jul;9(3):223-33.
Eight human corneal lenticules (three from keratophakia, two from hypermetropic keratomileusis, and three from myopic keratomileusis) were examined by light and electron microscopy. The keratophakic lenticules were removed for optical reasons 3, 3.5, and 6 months after surgery. Microscopically, all displayed hypocellularity, mature collagen fibrils and microfibrils, keratocyte ghosts, and keratocytic debris. The hypermetropic keratomileusis specimens were removed at 13 and 14 months postoperatively, the first because of opacities from enzymatic digestion of the cornea, and the second due to contact lens-induced erosion of Bowman's layer and decreased lacrimal secretion. Ultrastructurally, both lenticules exhibited fractures in Bowman's layer, and the 14-month specimen showed multilayered squamous epithelia. The myopic keratomileusis specimens were removed at 4, 8, and 48 months postoperatively due to opacification of the interface caused by delayed epithelial healing, detergent trauma, and previous epithelization, respectively. Electron microscopy revealed fractures in Bowman's layer, subepithelial fibrocellular growth, sparse keratocyte populations of the anterior stroma, porous collagen bundles, keratocytic debris, and regions of epithelial ingrowth.
对八个人类角膜透镜（三个来自角膜磨镶术，两个来自远视性角膜磨镶术，三个来自近视性角膜磨镶术）进行了光镜和电镜检查。角膜磨镶术的透镜在术后3、3.5和6个月因光学原因被取出。显微镜下，所有透镜均显示细胞减少、成熟的胶原纤维和微纤维、角膜细胞残影和角膜细胞碎片。远视性角膜磨镶术的标本在术后13和14个月被取出，第一个是因为角膜酶消化导致的混浊，第二个是由于隐形眼镜引起的Bowman层侵蚀和泪液分泌减少。超微结构上，两个透镜在Bowman层均出现断裂，14个月的标本显示有多层鳞状上皮。近视性角膜磨镶术的标本分别在术后4、8和48个月因上皮愈合延迟、去污剂损伤和先前的上皮化导致界面混浊而被取出。电子显微镜显示Bowman层断裂、上皮下纤维细胞生长、前基质角膜细胞数量稀少、多孔胶原束、角膜细胞碎片和上皮内生区域。
