Liu Xiaoping, Zweier Jay L
Davis Heart and Lung Research Institute, Department of Internal Medicine, The Ohio State University College of Medicine, 473 West 12th Avenue, Columbus, OH 43210, USA.
J Electroanal Chem (Lausanne). 2013 Jan 1;688:32-39. doi: 10.1016/j.jelechem.2012.09.038.
Nitric oxide (NO) has many important physiological roles in the body. Since NO electrodes can directly measure NO concentration in the nM range and in real time, NO electrode methods have been generally used in laboratories for measuring NO concentration in vivo and in vitro. This review focuses on the application of electrode methods in studies of NO diffusion and metabolic kinetics. We have described the physical and chemical properties that need to be considered in the preparation of NO stock solution, discussed the effect of several interfering factors on the measured curves of NO concentration that need to be eliminated in the experimental setup for NO measurements, and provided an overview of the application of NO electrode methods in measuring NO diffusion and metabolic kinetics in solution and in biological systems. This overview covers NO metabolism by oxygen (O), superoxide, heme proteins, cells and tissues. Important conclusions and physiological implication of these studies are discussed.
一氧化氮（NO）在体内具有许多重要的生理作用。由于NO电极能够直接实时测量纳摩尔范围内的NO浓度，因此NO电极法已普遍用于实验室中体内和体外NO浓度的测量。本综述重点关注电极法在NO扩散和代谢动力学研究中的应用。我们描述了制备NO储备溶液时需要考虑的物理和化学性质，讨论了几种干扰因素对NO浓度测量曲线的影响，这些因素在NO测量的实验装置中需要消除，并概述了NO电极法在测量溶液和生物系统中NO扩散和代谢动力学方面的应用。该概述涵盖了氧气（O）、超氧化物、血红素蛋白、细胞和组织对NO的代谢。讨论了这些研究的重要结论和生理意义。
