铜在抗坏血酸氧化酶对变性剂稳定性中的作用。
The role of copper in the stability of ascorbate oxidase towards denaturing agents.
作者信息
Savini I, D'Alessio S, Giartosio A, Morpurgo L, Avigliano L
机构信息
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma, Tor Vergata, Italy.
出版信息
Eur J Biochem. 1990 Jul 5;190(3):491-5. doi: 10.1111/j.1432-1033.1990.tb15600.x.
The susceptibility of native, type-2 Cu-depleted and fully Cu-depleted ascorbate oxidase to thermal and chemical denaturation has been probed by differential scanning calorimetry, fluorimetry and circular dichroism. The data indicate that copper affects the stability, but not the protein conformation. The unfolding of ascorbate oxidase is characterized by a single endotherm. Calorimetric domains revealed by deconvolution are consistent with the domains identified by X-ray crystallography.
通过差示扫描量热法、荧光法和圆二色性研究了天然的、2型铜缺乏型和完全铜缺乏型抗坏血酸氧化酶对热变性和化学变性的敏感性。数据表明铜会影响稳定性，但不影响蛋白质构象。抗坏血酸氧化酶的去折叠以单一吸热峰为特征。通过反卷积揭示的量热结构域与通过X射线晶体学鉴定的结构域一致。
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