晶状体甘油醛-3-磷酸脱氢酶的光氧化损伤及其与醛代谢的关系。

Photo-oxidative damage to lenticular GAPDH and its relationship to aldehyde metabolism.

作者信息

Jedziniak J

机构信息

Harvard Medical School, Massachusetts Eye and Ear Infirmary, Howe Laboratory of Ophthalmology, Boston 02114.

出版信息

Exp Eye Res. 1990 Jun;50(6):589-95. doi: 10.1016/0014-4835(90)90099-g.

DOI:10.1016/0014-4835(90)90099-g

PMID:2373157

Abstract

These studies show that GAPDH from the calf lens and human lens epithelium undergoes size and charge modifications in the presence of active oxygen species generated from methylene blue and light. The histidine content of the enzyme was reduced by one third. No evidence of increased proteolysis was observed suggesting a nonenzymatic cleavage of enzyme by the photo-oxidants.

摘要

这些研究表明，来自小牛晶状体和人晶状体上皮的甘油醛-3-磷酸脱氢酶（GAPDH）在亚甲蓝和光产生的活性氧存在下会发生大小和电荷的改变。该酶的组氨酸含量减少了三分之一。未观察到蛋白水解增加的迹象，这表明光氧化剂对酶进行了非酶促裂解。