Department of Veterinary Nursing and Technology, School of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
Vet J. 2013 Sep;197(3):769-75. doi: 10.1016/j.tvjl.2013.04.024. Epub 2013 May 31.
REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.
REIC/Dkk-3，一种人类 Dickkopf（Dkk）家族的成员，在多种人类癌症中扮演生长抑制因子的角色。在这项研究中，研究了犬类 REIC/Dkk-3 的肿瘤抑制功能。克隆了犬类 REIC/Dkk-3 同源物的全长开放阅读框，并确定了 REIC/Dkk-3 mRNA 的组织分布，以及 REIC/Dkk-3 蛋白在犬类癌细胞系中的亚细胞定位。与正常乳腺组织相比，REIC/Dkk-3 在乳腺肿瘤和犬乳腺癌细胞系中的表达水平较低。REIC/Dkk-3 的过表达诱导了犬乳腺癌细胞系的细胞凋亡。这些结果表明，REIC/Dkk-3 在犬乳腺肿瘤中的表达下调，该基因的功能之一是诱导细胞凋亡。
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