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犬REIC/Dkk-3与SGTA相互作用，并在雄激素非依赖性前列腺癌细胞系中恢复雄激素受体信号传导。

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

作者信息

Kato Yuiko, Ochiai Kazuhiko, Kawakami Shota, Nakao Nobuhiro, Azakami Daigo, Bonkobara Makoto, Michishita Masaki, Morimatsu Masami, Watanabe Masami, Omi Toshinori

机构信息

School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, 180-8602, Japan.

Laboratory of Animal Physiology, School of Animal Science, Nippon Veterinary and Life Science University, Tokyo, 180-8602, Japan.

出版信息

BMC Vet Res. 2017 Jun 9;13(1):170. doi: 10.1186/s12917-017-1094-4.

DOI:10.1186/s12917-017-1094-4
PMID:28599655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5466802/
Abstract

BACKGROUND

The pathological condition of canine prostate cancer resembles that of human androgen-independent prostate cancer. Both canine and human androgen receptor (AR) signalling are inhibited by overexpression of the dimerized co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is considered to cause the development of androgen-independency. Reduced expression in immortalised cells (REIC/Dkk-3) interferes with SGTA dimerization and rescues AR signalling. This study aimed to assess the effects of REIC/Dkk-3 and SGTA interactions on AR signalling in the canine androgen-independent prostate cancer cell line CHP-1.

RESULTS

Mammalian two-hybrid and Halo-tagged pull-down assays showed that canine REIC/Dkk-3 interacted with SGTA and interfered with SGTA dimerization. Additionally, reporter assays revealed that canine REIC/Dkk-3 restored AR signalling in both human and canine androgen-independent prostate cancer cells. Therefore, we confirmed the interaction between canine SGTA and REIC/Dkk-3, as well as their role in AR signalling.

CONCLUSIONS

Our results suggest that this interaction might contribute to the development of a novel strategy for androgen-independent prostate cancer treatment. Moreover, we established the canine androgen-independent prostate cancer model as a suitable animal model for the study of this type of treatment-refractory human cancer.

摘要

背景

犬前列腺癌的病理状况与人类雄激素非依赖性前列腺癌相似。犬和人类的雄激素受体（AR）信号传导均受到二聚化共伴侣富含谷氨酰胺的小四肽重复序列蛋白α（SGTA）过表达的抑制，这被认为会导致雄激素非依赖性的发展。永生化细胞中表达降低的蛋白（REIC/Dkk-3）会干扰SGTA二聚化并挽救AR信号传导。本研究旨在评估REIC/Dkk-3与SGTA相互作用对犬雄激素非依赖性前列腺癌细胞系CHP-1中AR信号传导的影响。

结果

哺乳动物双杂交和 Halo 标签下拉试验表明，犬REIC/Dkk-3与SGTA相互作用并干扰SGTA二聚化。此外，报告基因试验显示，犬REIC/Dkk-3在人类和犬雄激素非依赖性前列腺癌细胞中均恢复了AR信号传导。因此，我们证实了犬SGTA与REIC/Dkk-3之间的相互作用及其在AR信号传导中的作用。

结论

我们的结果表明，这种相互作用可能有助于开发一种治疗雄激素非依赖性前列腺癌的新策略。此外，我们建立了犬雄激素非依赖性前列腺癌模型，作为研究这种难治性人类癌症的合适动物模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/cf112f93a2a0/12917_2017_1094_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/4f80f5accd38/12917_2017_1094_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/f73e1a6ec8c4/12917_2017_1094_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/edec0181ccee/12917_2017_1094_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/cf112f93a2a0/12917_2017_1094_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/4f80f5accd38/12917_2017_1094_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/f73e1a6ec8c4/12917_2017_1094_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/edec0181ccee/12917_2017_1094_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51c8/5466802/cf112f93a2a0/12917_2017_1094_Fig4_HTML.jpg

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本文引用的文献

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Vet Comp Oncol. 2017 Jun;15(2):557-562. doi: 10.1111/vco.12199. Epub 2016 Jan 14.
2
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Oncotarget. 2016 Jan 19;7(3):3283-96. doi: 10.18632/oncotarget.6488.
3
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细胞质质量控制蛋白 SGTA 和 BAG6 复合物在疾病中的作用。
Adv Protein Chem Struct Biol. 2019;114:265-313. doi: 10.1016/bs.apcsb.2018.11.002. Epub 2018 Dec 18.
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Vet J. 2015 Nov;206(2):143-8. doi: 10.1016/j.tvjl.2015.08.002. Epub 2015 Aug 7.
4
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5
Global cancer statistics, 2012.全球癌症统计数据，2012 年。
CA Cancer J Clin. 2015 Mar;65(2):87-108. doi: 10.3322/caac.21262. Epub 2015 Feb 4.
6
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CA Cancer J Clin. 2015 Jan-Feb;65(1):5-29. doi: 10.3322/caac.21254. Epub 2015 Jan 5.
7
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J Cell Biochem. 2014 Jan;115(1):141-50. doi: 10.1002/jcb.24641.