Zhang Yu-Ting, Zhou De-Qun, Su Yuan, Yu Ping, Zhou Xiao-Gang, Yao Chun-Xin
Yunnan Academy of Agricultural Sciences, Kunming, China.
Yi Chuan. 2013 May;35(5):666-72. doi: 10.3724/sp.j.1005.2013.00666.
It is utmost important to cultivate potato variety with drought resistance to reduce arid loss by research progress on potato drought resistance molecular mechanism. A comparative study with differences in protein group analysis of potato drought resistance variety in Ninglang 182 leaves was carried out by using two-dimensional gel electrophoresis during drought and normal processing conditions.There were 12 differentially expressed protein spots identified by Electro-phoresis and MALDI-TOF-TOF/MS analysis. The function classification of these proteins results that the potato varieties to drought tolerance could be improved through the protection of photosynthesis and mitochondria, regulation of the signal transduction induced under environmental stress and regulation of plant tissue N and C transport system, these proteins expression were increased under drought.The results showed that these proteins are the drought resistance associated proteins of potato variety in Ninglang 182.This study provided a theoretical basis of the molecular mechanism of improving drought tolerance in order to expound the potato drought resistance variety through multiple paths and the level regulation.
通过对马铃薯抗旱分子机制的研究进展，培育抗旱马铃薯品种以减少干旱损失至关重要。利用双向凝胶电泳技术，对宁蒗182叶片在干旱和正常处理条件下的抗旱品种进行了蛋白质组分析差异的比较研究。通过电泳和基质辅助激光解吸电离飞行时间串联质谱分析，鉴定出12个差异表达蛋白点。这些蛋白质的功能分类结果表明，马铃薯品种的耐旱性可通过保护光合作用和线粒体、调节环境胁迫诱导的信号转导以及调节植物组织氮和碳运输系统来提高，这些蛋白质在干旱条件下表达增加。结果表明，这些蛋白质是宁蒗182马铃薯品种的抗旱相关蛋白。本研究为阐明马铃薯抗旱品种通过多种途径和水平调控提高耐旱性的分子机制提供了理论依据。
