Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec H3A 2B2, Canada.
Colloids Surf B Biointerfaces. 2013 Oct 1;110:275-80. doi: 10.1016/j.colsurfb.2013.03.047. Epub 2013 Apr 29.
The motility of bacteria plays a key role in their colonization of surfaces during infection. Derivatives of cranberry fruit have been shown to interfere with bacterial motility. Herein, we report on the incorporation of cranberry derived materials (CDMs) into silicone substrates with the aim of impairing bacterial pathogen motility and spreading on the substrate surface. The release of CDMs from the silicone substrates when soaking in an aqueous medium was quantified for a period of 24h. Next, we showed that CDMs released from two silicone substrates remain bioactive as they downregulate the expression of the flagellin gene of two key uropathogens - Escherichia coli CFT073 and Proteus mirabilis HI4320. Furthermore, we demonstrate that CDM-modified silicone inhibits the swarming motility of P. mirabilis, an aggressive swarmer. The bioactive, CDM-modified substrates can find broad applications in the medical device and food industries where the impairment of bacterial colonization of surfaces is of paramount importance.
细菌的运动性在感染过程中其对表面的定殖起着关键作用。蔓越莓果实的衍生物已被证明可以干扰细菌的运动性。在此，我们报告了将蔓越莓衍生材料 (CDM) 掺入硅酮基质中，以削弱细菌病原体在基质表面的运动性和扩散性。在水性介质中浸泡 24 小时后，定量测定了硅酮基质中 CDM 的释放情况。接下来，我们表明，从两种硅酮基质中释放的 CDM 仍然具有生物活性，因为它们下调了两种关键尿路病原体 - 大肠杆菌 CFT073 和奇异变形杆菌 HI4320 的鞭毛基因的表达。此外，我们证明 CDM 修饰的硅酮抑制了侵袭性游泳者奇异变形杆菌的群集运动性。具有生物活性的、CDM 修饰的基质可以在医疗器械和食品工业中得到广泛应用，因为这些工业中表面细菌定殖的削弱至关重要。
