通过 SPR、CV 和紫外可见光谱研究阳离子卟啉与 ctDNA 的相互作用。
Interaction between a cationic porphyrin and ctDNA investigated by SPR, CV and UV-vis spectroscopy.
机构信息
State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
出版信息
Colloids Surf B Biointerfaces. 2013 Oct 1;110:321-6. doi: 10.1016/j.colsurfb.2013.04.040. Epub 2013 Apr 30.
The interaction between ctDNA and a cationic porphyrin was studied in this work. The binding process was monitored by surface plasmon resonance (SPR) spectroscopy in detail. The association, dissociation rate constants and the binding constants calculated by global analysis were 2.4×10(2)±26.4M(-1)s(-1), 0.011±0.0000056s(-1) and 2.18×10(4)M(-1), respectively. And the results were confirmed by cyclic voltammetry and UV-vis absorption spectroscopy. The binding constants obtained from cyclic voltammetry and UV-vis absorption spectroscopy were 8.28×10(4)M(-1) and 6.73×10(4)M(-1) at 298K, respectively. The covalent immobilization methodology of ctDNA onto gold surface modified with three different compounds was also investigated by SPR. These compounds all contain sulfydryl but with different terminated functional groups. The results indicated that the 11-MUA (HS(CH2)10COOH)-modified gold film is more suitable for studying the DNA-drug interaction.
本工作研究了 ctDNA 与阳离子卟啉之间的相互作用。通过表面等离子体共振（SPR）光谱详细监测了结合过程。通过全局分析计算得出的结合、解离速率常数和结合常数分别为 2.4×10(2)±26.4M(-1)s(-1)、0.011±0.0000056s(-1)和 2.18×10(4)M(-1)。并通过循环伏安法和紫外-可见吸收光谱进行了验证。在 298K 时，循环伏安法和紫外-可见吸收光谱得到的结合常数分别为 8.28×10(4)M(-1)和 6.73×10(4)M(-1)。还通过 SPR 研究了三种不同化合物修饰的金表面上 ctDNA 的共价固定方法。这些化合物都含有巯基，但具有不同的末端官能团。结果表明，11-MUA（HS(CH2)10COOH）修饰的金膜更适合研究 DNA-药物相互作用。
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