两种含5-氟尿嘧啶衍生物的半夹心钌（II）化合物：DNA嵌入的合成与研究
Two half-sandwiched ruthenium (II) compounds containing 5-fluorouracil derivatives: synthesis and study of DNA intercalation.
作者信息
Li Zhao-Jun, Hou Yong, Qin Da-An, Jin Zhi-Min, Hu Mao-Lin
机构信息
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, China.
Institute of Biotechnology and Nucleic Technology, Sichuan Academy of Agricultural Sciences, Chengdu, China.
出版信息
PLoS One. 2015 Mar 19;10(3):e0120211. doi: 10.1371/journal.pone.0120211. eCollection 2015.
Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements. The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.
合成了两种含2-(5-氟尿嘧啶)-N-(吡啶基)-乙酰胺的半夹心钌(II)新型配位化合物，通过紫外可见光谱的增色效应揭示了它们与小牛胸腺DNA的嵌入结合模式；在仔细的循环伏安法测量基础上推导出朗缪尔吸附方程，从而确定了结合常数。这两种化合物均表现出DNA嵌入结合活性，结合常数分别为1.13×10⁶ M⁻¹和5.35×10⁵ M⁻¹。
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