Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, (CINVESTAV-IPN); Irapuato-León; Irapuato, Gto., México.
Plant Signal Behav. 2013 Aug;8(8). doi: 10.4161/psb.25160. Epub 2013 Jun 3.
After fertilization in Arabidopsis, auxin response in ovules triggers fruit development through the stimulation of gibberellin metabolism. In a recent work, we showed that this model could not explain why CYP78A9 overexpression can uncouple these processes. The specific expression pattern of CYP78A9 suggests its involvement during reproductive development. Moreover, controlled pollination showed that CYP78A9 responds to fertilization. The genetic evidence supports the idea that CYP78A9 and its closest paralogs participate in a pathway that control floral organ size and ovule integuments development as denoted by the phenotypes of es1-D overexpression and cyp78a8 cyp78a9 double mutants. Furthermore, according to previous predictions, perturbations in the flavonol biosynthesis pathway were detected in cyp78a9, cyp78a8 cyp78a9 and es1-D mutants. However, they do not cause the observed phenotypes. Our results add new insights into the role of CYP78A9 in plant reproduction and present the first characterization of metabolite differences between mutants in this gene family.
在拟南芥受精后，胚珠中的生长素反应通过刺激赤霉素代谢来触发果实发育。在最近的一项工作中，我们表明，这个模型无法解释为什么 CYP78A9 的过表达可以使这些过程脱耦。CYP78A9 的特定表达模式表明其参与了生殖发育过程。此外，受控授粉表明 CYP78A9 对受精有反应。遗传证据支持这样一种观点，即 CYP78A9 及其最接近的同源物参与了控制花器官大小和胚珠被发育的途径，这一点可以从 es1-D 过表达和 cyp78a8 cyp78a9 双突变体的表型中看出。此外，根据之前的预测，在 cyp78a9、cyp78a8 cyp78a9 和 es1-D 突变体中检测到类黄酮生物合成途径的扰动，但这些突变体并没有引起观察到的表型。我们的研究结果为 CYP78A9 在植物生殖中的作用提供了新的见解，并首次对该基因家族中突变体之间代谢物差异进行了表征。
