Janko Christina, Munoz Luis, Chaurio Ricardo, Maueröder Christian, Berens Christian, Lauber Kirsten, Herrmann Martin
Department of Internal Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Methods Mol Biol. 2013;1004:3-15. doi: 10.1007/978-1-62703-383-1_1.
Apoptosis and necrosis reflect the program of cell death employed by a dying cell and the final stage of death, respectively. Whereas apoptosis is defined as a physiological, highly organized cell death process, necrosis is commonly considered to be accidental and uncontrolled. Physiological and weak pathological death stimuli preferentially induce apoptosis, while harsh non-physiological insults often immediately instigate (primary) necrosis. If an apoptosing cell transits into a phase of plasma membrane disintegration, this stage of death is referred to as secondary or post-apoptotic necrosis.Here, we present several conditions that stimulate primary and/or secondary necrosis and show that necrosis displays considerably different time courses. For subclassification of necrotic phenotypes we employed a flow cytometric single-tube 4-color staining technique including annexin A5-FITC, propidium iodide, DiIC1(5), and Hoechst 33342.
凋亡和坏死分别反映了垂死细胞所采用的细胞死亡程序和死亡的最终阶段。凋亡被定义为一种生理性的、高度有序的细胞死亡过程，而坏死通常被认为是意外的和不受控制的。生理性和轻度病理性死亡刺激优先诱导凋亡，而强烈的非生理性损伤往往立即引发（原发性）坏死。如果一个正在凋亡的细胞进入质膜崩解阶段，这个死亡阶段被称为继发性或凋亡后坏死。在这里，我们展示了几种刺激原发性和/或继发性坏死的条件，并表明坏死表现出明显不同的时间进程。为了对坏死表型进行亚分类，我们采用了一种流式细胞术单管四色染色技术，包括膜联蛋白A5-异硫氰酸荧光素、碘化丙啶、二碘荧光素1(5)和 Hoechst 33342。
