Dmitrenko V V, Kavsan V M
Genetika. 1990 Apr;26(4):765-9.
Human fetal liver cDNA was cloned in pBR322 vector by dG:dC-tailing method. The cDNA library was screened for liver-specific clones by means of differential hybridization. Human fetal liver and human kidney cDNAs were used as hybridization probes. Application of this procedure revealed twenty five liver-specific clones among about one thousand recombinants analysed. These clones represent cDNAs corresponding abundant mRNAs. Eighteen clones were identified as encoding serum albumin. Two different mRNA polyadenylation sites were found in four sequenced plasmids. Cleavage/polyadenylation site in two plasmids, pHA1 and pHA12, is situated fifteen nucleotides downstream the AATAAA signal; in two other plasmids, pHA8 and pHA25, this site is ten nucleotides downstream the same signal.
人胎儿肝脏cDNA通过dG:dC加尾法克隆于pBR322载体。通过差异杂交筛选该cDNA文库以获得肝脏特异性克隆。人胎儿肝脏和人肾脏cDNA用作杂交探针。应用此方法在分析的约一千个重组体中揭示了二十五个肝脏特异性克隆。这些克隆代表了与丰富mRNA相对应的cDNA。十八个克隆被鉴定为编码血清白蛋白。在四个测序质粒中发现了两个不同的mRNA聚腺苷酸化位点。两个质粒pHA1和pHA12中的切割/聚腺苷酸化位点位于AATAAA信号下游十五个核苷酸处；在另外两个质粒pHA8和pHA25中，该位点位于相同信号下游十个核苷酸处。
