使用HeLa细胞核提取物分析前体mRNA剪接
Analysis of pre-mRNA splicing using HeLa cell nuclear extracts.
作者信息
Nilsen Timothy W
出版信息
Cold Spring Harb Protoc. 2013 Jun 1;2013(6):584-7. doi: 10.1101/pdb.prot075184.
PMID:23734029
Abstract
This protocol is used to determine the splicing behavior of pre-mRNAs in cell extracts that are capable of carrying out splicing (e.g., nuclear extracts from HeLa cells). (32)P-labeled RNA is incubated under splicing conditions for various times, and the resulting products are analyzed on denaturing polyacrylamide gels.
摘要
该方案用于确定能够进行剪接的细胞提取物（例如来自HeLa细胞的核提取物）中前体mRNA的剪接行为。将用（32）P标记的RNA在剪接条件下孵育不同时间，然后在变性聚丙烯酰胺凝胶上分析所得产物。
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