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大麦粒硫氧还蛋白系统——更新。

The barley grain thioredoxin system - an update.

机构信息

Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark Kongens Lyngby, Denmark.

出版信息

Front Plant Sci. 2013 May 21;4:151. doi: 10.3389/fpls.2013.00151. eCollection 2013.

DOI:10.3389/fpls.2013.00151
PMID:23734159
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3659290/
Abstract

Thioredoxin (Trx) reduces disulfide bonds and play numerous important functions in plants. In cereal seeds, cytosolic h-type Trx facilitates the release of energy reserves during the germination process and is recycled by NADPH-dependent Trx reductase. This review presents a summary of the research conducted during the last 10 years to elucidate the structure and function of the barley seed Trx system at the molecular level combined with proteomic approaches to identify target proteins.

摘要

硫氧还蛋白(Trx)可以还原二硫键,并在植物中发挥许多重要功能。在谷类种子中,细胞质 h 型 Trx 有助于在萌发过程中释放能量储备,并通过 NADPH 依赖的 Trx 还原酶进行循环利用。本文综述了过去 10 年的研究工作,从分子水平阐明了大麦种子 Trx 系统的结构和功能,并结合蛋白质组学方法鉴定了靶蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1507/3659290/7166dd388597/fpls-04-00151-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1507/3659290/90657eead171/fpls-04-00151-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1507/3659290/7166dd388597/fpls-04-00151-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1507/3659290/90657eead171/fpls-04-00151-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1507/3659290/7166dd388597/fpls-04-00151-g002.jpg

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Biochemistry. 2012 Dec 11;51(49):9930-9. doi: 10.1021/bi301051b. Epub 2012 Nov 29.
2
Inactivation of barley limit dextrinase inhibitor by thioredoxin-catalysed disulfide reduction.硫氧还蛋白催化的二硫键还原使大麦极限糊精酶抑制剂失活。
FEBS Lett. 2012 Jul 30;586(16):2479-82. doi: 10.1016/j.febslet.2012.06.009. Epub 2012 Jun 19.
3
Kinetic and thermodynamic properties of two barley thioredoxin h isozymes, HvTrxh1 and HvTrxh2.
大麦胚乳发育过程中质体淀粉磷酸化酶的功能与结构特征
PLoS One. 2017 Apr 13;12(4):e0175488. doi: 10.1371/journal.pone.0175488. eCollection 2017.
两种大麦硫氧还蛋白 h 同工酶(HvTrxh1 和 HvTrxh2)的动力学和热力学性质。
FEBS Lett. 2010 Aug 4;584(15):3376-80. doi: 10.1016/j.febslet.2010.06.028. Epub 2010 Jun 23.
4
Identification of thioredoxin target disulfides in proteins released from barley aleurone layers.鉴定从小麦糊粉层中释放的蛋白质中的硫氧还蛋白靶二硫键。
J Proteomics. 2010 Apr 18;73(6):1133-6. doi: 10.1016/j.jprot.2010.01.007. Epub 2010 Jan 28.
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