Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark Kongens Lyngby, Denmark.
Front Plant Sci. 2013 May 21;4:151. doi: 10.3389/fpls.2013.00151. eCollection 2013.
Thioredoxin (Trx) reduces disulfide bonds and play numerous important functions in plants. In cereal seeds, cytosolic h-type Trx facilitates the release of energy reserves during the germination process and is recycled by NADPH-dependent Trx reductase. This review presents a summary of the research conducted during the last 10 years to elucidate the structure and function of the barley seed Trx system at the molecular level combined with proteomic approaches to identify target proteins.
硫氧还蛋白(Trx)可以还原二硫键,并在植物中发挥许多重要功能。在谷类种子中,细胞质 h 型 Trx 有助于在萌发过程中释放能量储备,并通过 NADPH 依赖的 Trx 还原酶进行循环利用。本文综述了过去 10 年的研究工作,从分子水平阐明了大麦种子 Trx 系统的结构和功能,并结合蛋白质组学方法鉴定了靶蛋白。