重组表达异源草酸脱羧酶的植物乳杆菌体外降解草酸。
In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase.
机构信息
Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, India.
出版信息
J Appl Microbiol. 2013 Sep;115(3):880-7. doi: 10.1111/jam.12269. Epub 2013 Jun 24.
AIM
The aim of the present study is to constitutively express heterologous oxalate decarboxylase (OxdC) in Lactobacillus plantarum and to examine its ability to degrade oxalate in vitro for their future therapy against enteric hyperoxaluria.
METHOD AND RESULTS
In this study, we generated a recombinant strain of Lb. plantarum to constitutively overexpress B. subtilis oxalate decarboxylase (oxdC) using a host lactate dehydrogenase promoter (PldhL ). The recombinant Lb. plantarum was able to degrade more than 90% oxalate compared to 15% by the wild type. In addition, the recombinant strain also had higher tolerance up to 500 mmol l(-1) oxalate.
CONCLUSION
We developed a recombinant Lb. plantarum NC8 that constitutively expressed heterologous oxalate decarboxylase and degraded oxalate efficiently under in vitro conditions.
SIGNIFICANCE AND IMPACT OF STUDY
The long-term aim is to develop an efficient strain for future therapy against oxalosis.
目的
本研究旨在构建能够稳定表达异源草酸脱羧酶（OxdC）的植物乳杆菌，并检测其在体外降解草酸的能力，以期为治疗肠道高草酸尿症提供新方法。
方法和结果
本研究利用枯草芽孢杆菌草酸脱羧酶（oxdC）的启动子（PldhL）构建了一株植物乳杆菌重组菌株，以实现 oxdC 的稳定过表达。与野生型相比，重组植物乳杆菌能够降解超过 90%的草酸，而野生型仅能降解 15%。此外，该重组菌株对草酸的耐受能力也更高，可达 500mmol/L。
结论
我们成功构建了一株能够稳定表达异源草酸脱羧酶的植物乳杆菌 NC8 重组菌株，该菌株能够在体外条件下有效降解草酸。
研究意义和影响
我们的长期目标是开发一种高效的菌株，用于治疗草酸钙结石症。
Zotero 插件