Department of Rheumatology, Aarhus University Hospital, Aarhus, Denmark.
J Microsc. 2013 Aug;251(2):133-43. doi: 10.1111/jmi.12055. Epub 2013 Jun 4.
Design-based stereological methods using systematic uniform random sampling, the Cavalieri estimator and vertical sections are used to investigate undecalcified human femoral heads. Ten entire human femoral heads, obtained from normal women and normal men, were systematically sampled and thin undecalcified vertical sections were obtained. Absolute volumes and surface areas of the entire femoral head, the articular cartilage and the calcified cartilage compartments were estimated. In addition, the average thickness of the articular cartilage and the calcified cartilage were calculated. The stereological procedures applied to the human femoral heads resulted in average coefficient of errors, which were 0.03-0.06 for the volume estimates and 0.03-0.04 for the surface area estimates. We conclude that design-based stereology using the Cavalieri estimator and vertical sections can successfully be used in large undecalcified tissue specimens, like the human femoral head, to estimate the absolute volume and surface area of macroscopic as well as of microscopic tissue compartments. The application of well-known design-based stereological methods carries potential advantage for investigating the pathology in inflammatory and degenerative joint diseases.
基于设计的体视学法采用系统均匀随机抽样、Cavalieri 估计量和垂直切片，用于研究未脱钙的人股骨头。从正常女性和正常男性中获得的 10 个人类完整股骨头被系统地取样，并获得了薄的未脱钙垂直切片。估计了整个股骨头、关节软骨和钙化软骨隔室的绝对体积和表面积。此外，还计算了关节软骨和钙化软骨的平均厚度。应用于人股骨头的体视学程序导致平均误差系数为 0.03-0.06，用于体积估计，0.03-0.04 用于表面积估计。我们得出结论，使用 Cavalieri 估计量和垂直切片的基于设计的体视学法可以成功地用于大型未脱钙组织标本，如人股骨头，以估计宏观和微观组织隔室的绝对体积和表面积。应用知名的基于设计的体视学方法可能有助于研究炎症和退行性关节疾病的病理学。
