利用荧光探针对链霉亲和素的简便表征，实现链霉亲和素在磁性亚微米颗粒上的固定。
Facile characterization of the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidin.
机构信息
Unit for Analytical Probes and Protein Biotechnology, Key Laboratory of Clinical Laboratory Diagnostics of the Education Ministry, College of Laboratory Medicine, Chongqing Medical University, Chongiqng 400016, China.
出版信息
Appl Spectrosc. 2013 Jun;67(6):688-91. doi: 10.1366/12-06963.
To characterize streptavidin immobilization on magnetic submicron particles (MSPs), residual streptavidin after magnetic removal of immobilized streptavidin was quantified with N-biotinyl-N'-(1-naphthyl)-ethylenediamine (BNEDA) based on Förster resonance energy transfer. Residual BNEDA after magnetic removal of bound BNEDA was measured by its own fluorescence. Streptavidin was immobilized at about 12 mg per gram of MSPs and easily retained over 50% of its original activity. These assays facilitated optimized streptavidin immobilization on MSPs.
为了表征链霉亲和素在磁性亚微米颗粒（MSP）上的固定化，使用基于Förster 共振能量转移的 N-生物素基-N'-(1-萘基）乙二胺（BNEDA）定量测定固定化链霉亲和素经磁去除后的残留链霉亲和素。通过其自身的荧光测量结合 BNEDA 经磁去除后的残留 BNEDA。链霉亲和素的固定化量约为每克 MSP 12 毫克，并且容易保留其原始活性的 50%以上。这些测定方法促进了链霉亲和素在 MSP 上的优化固定化。
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