Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Biomed Mater. 2013 Aug;8(4):045005. doi: 10.1088/1748-6041/8/4/045005. Epub 2013 Jun 4.
An in vitro model that recapitulates the characteristics of native human adipose tissue would largely benefit pathology studies and therapy development. In this paper, we fabricated a physiological model composed of both human adipocytes and endothelial cells with spatially controlled distribution that biomimics the structure and composition of human adipose tissue. Detailed studies into the cell-cell interactions between the adipocytes and endothelial cells revealed a mutual-enhanced effect which resembles the in vivo routine. Furthermore, comparisons between planar coculture and model coculture demonstrated improved adipocyte function as well as endothelial cell proliferation under the same conditions. This research provided a reliable model for human adipose tissue development studies and potential obesity-related therapy development.
一种能够重现天然人脂肪组织特征的体外模型将极大地有益于病理学研究和治疗方法的开发。在本文中，我们构建了一种由人脂肪细胞和内皮细胞组成的生理模型，其具有空间控制的分布，模拟了人脂肪组织的结构和组成。对脂肪细胞和内皮细胞之间的细胞-细胞相互作用的详细研究揭示了一种类似于体内常规的相互增强效应。此外，在相同条件下，将平面共培养和模型共培养进行比较，结果表明模型共培养能够改善脂肪细胞功能和内皮细胞增殖。这项研究为人类脂肪组织发育研究和潜在的肥胖相关治疗方法的开发提供了可靠的模型。
