Department of Biomedical Sciences, New York Institute of Technology, College of Osteopathic Medicine, Old Westbury, NY 11568-8000, USA.
Brain Res Bull. 2013 Aug;97:63-8. doi: 10.1016/j.brainresbull.2013.05.001. Epub 2013 Jun 2.
C57BL/6 mice exhibit spontaneous cerebellar malformations consisting of heterotopic neurons and glia in the molecular layer of the vermis (Tanaka and Marunouchi, 2005; Mangaru et al., 2013). Malformations are only found between folia VIII and IX and are indicative of deficits of neuronal migration during cerebellar development. In the present report we test the prediction that mutant and transgenic mouse models on a C57BL/6 background will also exhibit these same cerebellar malformations. Consistent with our hypothesis, we found that 2 spontaneous mutant models of Parkinson's disease on a C57BL/6 background had cerebellar malformations. In addition, we found that numerous transgenic mouse lines on a full or partial C57BL/6 background including eGFP-, YFP- and Cre-transgenic mice also exhibited heterotopia. These data suggest that histological analyses be performed in studies of cerebellar function or development when using C57BL/6 or other mice on this background in order for correct interpretation of research results.
C57BL/6 小鼠表现出自发的小脑畸形，包括蚓部分子层中的异位神经元和神经胶质（Tanaka 和 Marunouchi，2005；Mangaru 等人，2013）。畸形仅发生在 VIII 叶和 IX 叶之间，表明小脑发育过程中神经元迁移不足。在本报告中，我们检验了这样一个预测，即在 C57BL/6 背景下的突变体和转基因小鼠模型也将表现出这些相同的小脑畸形。与我们的假设一致，我们发现 2 种自发的帕金森病突变体模型在 C57BL/6 背景下存在小脑畸形。此外，我们发现许多在全或部分 C57BL/6 背景下的转基因小鼠系，包括 eGFP-、YFP- 和 Cre-转基因小鼠，也表现出异位。这些数据表明，在使用 C57BL/6 或其他背景下的小鼠进行小脑功能或发育研究时，应进行组织学分析，以便正确解释研究结果。
