荧光光谱电化学法测定活性氧探针的灵敏度。
Sensitivity of activatable reactive oxygen species probes by fluorescence spectroelectrochemistry.
机构信息
Washington University School of Medicine, Department of Radiology, 510 S. Kingshighway, St. Louis, MO 63110, USA.
出版信息
Analyst. 2013 Aug 7;138(15):4363-9. doi: 10.1039/c3an00459g. Epub 2013 Jun 4.
Abstract
We have developed a new analytical method of evaluating activatable fluorescent probes for ROS detection using integrated fluorescence spectroelectrochemistry. The Tafel formalism was applied to describe the process of the probes' oxidation under electrochemical conditions and identify a novel parameter defined as the threshold oxidation potential. This potential can serve as an approximation to the equilibrium potential and can be utilized for determining the sensitivity of a probe to oxidation. Based upon the measured values of threshold potentials, the order of sensitivity towards oxidation among several commonly used probes was determined to be the following (from highest to lowest): 2,7-dihydrodichlorofluorescein > dihydroethidium > dihydrorhodamine 123 > dihydrorhodamine 6G. The presented approach opens up a new direction in synthesizing and screening novel ROS probes with a well-defined sensitivity for in vitro and in vivo applications.
摘要
我们开发了一种新的分析方法，用于使用集成荧光光谱电化学评估用于 ROS 检测的可活化荧光探针。塔菲尔公式被应用于描述探针在电化学条件下的氧化过程，并确定了一个新的参数，定义为阈值氧化电位。该电位可近似为平衡电位，并可用于确定探针对氧化的灵敏度。基于测量的阈值电位值，确定了几种常用探针的氧化灵敏度顺序为（从高到低）：2,7-二氢二氯荧光素>二氢乙啶>二氢罗丹明 123>二氢罗丹明 6G。所提出的方法为体外和体内应用中合成和筛选具有明确定义灵敏度的新型 ROS 探针开辟了一个新的方向。
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