Ramírez-Mendiola B, Flores-Pérez C, García-Álvarez R, Juárez-Olguín H, Flores-Pérez J, Chávez Pacheco J L
Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico.
Drug Res (Stuttg). 2013 Sep;63(9):473-6. doi: 10.1055/s-0033-1345183. Epub 2013 Jun 4.
The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml-1 and had correlation coefficients of r=0.995 and r=0.997 for sildenafil and its metabolite, respectively. The inter- and intra-day coefficients of variation was<5.3%. The limits of detection and quantification were 1 and 10 ng · ml-1. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed for use in children with Pulmonary Arterial Hypertension under treatment with sildenafil.
本研究的目的是开发一种简单的方法，通过使用C18反相柱和紫外检测的液相色谱法来定量西地那非及其代谢物的血浆水平。对于这两种化合物，在10至1000 ng·ml-1范围内评估线性，西地那非及其代谢物的相关系数分别为r = 0.995和r = 0.997。日内和日间变异系数均<5.3%。检测限和定量限分别为1和10 ng·ml-1。在两名患者中令人满意地测定了药物水平。开发了一种简单可靠的方法，用于接受西地那非治疗的肺动脉高压儿童。
