Li Yongjun, Tian Dean
Department of Gastroenterology, The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang 832006;
Exp Ther Med. 2013 May;5(5):1456-1460. doi: 10.3892/etm.2013.983. Epub 2013 Feb 28.
The aim of this study was to investigate the correlation between the expression of transforming growth factor-β1 (TGF-β1) in the mucosal tissue of rat small intestine and the apoptosis of epithelial cells in the small intestine in a cold-restraint stress state. Immunohistochemistry was used to detect the expression of TGF-β1. Terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) and DNA agarose gel electrophoresis were used to detect apoptosis. After 8 and 12 h of cold-restraint stress, the positive expression rate of TGF-β1 in the rat small intestine epithelial tissue was 59.09 and 54.16%, respectively. The apoptotic index (AI) of the rat small intestine epithelial cells was 25.69±8.09 and 19.65±6.61%, respectively. The positive expression rate of TGF-β1 in the epithelial tissue of the rat small intestine was positively correlated with the AI of the epithelial cells (r=0.980, P<0.05). The epithelial cells of the rat small intestine exhibited apoptosis under cold-restraint stress. TGF-β1 is one of the key factors that induces apoptosis of the epithelial cells of the rat small intestine.
本研究旨在探讨冷束缚应激状态下大鼠小肠黏膜组织中转化生长因子-β1（TGF-β1）的表达与小肠上皮细胞凋亡之间的相关性。采用免疫组织化学法检测TGF-β1的表达。运用末端脱氧核苷酸转移酶脱氧尿苷三磷酸（dUTP）缺口末端标记法（TUNEL）及DNA琼脂糖凝胶电泳检测细胞凋亡情况。冷束缚应激8 h和12 h后，大鼠小肠上皮组织中TGF-β1的阳性表达率分别为59.09%和54.16%。大鼠小肠上皮细胞的凋亡指数（AI）分别为25.69±8.09%和19.65±6.61%。大鼠小肠上皮组织中TGF-β1的阳性表达率与上皮细胞的AI呈正相关（r=0.980，P<0.05）。冷束缚应激状态下大鼠小肠上皮细胞出现凋亡。TGF-β1是诱导大鼠小肠上皮细胞凋亡的关键因素之一。
