Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou, 215006, PR China.
Langmuir. 2013 Jun 25;29(25):8039-45. doi: 10.1021/la4015652. Epub 2013 Jun 14.
Cellular uptake of materials plays a key role in their biomedical applications. In this work, based on the cell-mimic giant unilamellar vesicles (GUVs) and a novel type of microscale materials consisting of stimuli-responsive poly(N-isopropylacrylamide) microgel particles and the incorporated lipids, the influence of particle surface chemistry, including hydrophobic/hydrophilic property and lipid decorations, on the adsorption and consequent internalization of particles into GUVs was investigated. It is found that the decoration of particle surface with lipids facilitates the adsorption of particles on GUV membrane. After that, the hydrophobic property of particle surface further triggers the internalization of particles into GUVs. These results demonstrate the importance of surface properties of particles on their interactions with lipid membranes and are helpful to the understanding of cellular uptake mechanism.
细胞对材料的摄取在其生物医学应用中起着关键作用。在这项工作中，基于模拟细胞的巨大单层囊泡（GUVs）和一种新型微尺度材料，由对刺激响应的聚（N-异丙基丙烯酰胺）微凝胶颗粒和掺入的脂质组成，研究了颗粒表面化学性质，包括疏水性/亲水性和脂质修饰，对颗粒在 GUVs 中的吸附和随之发生的内化的影响。结果发现，颗粒表面用脂质修饰有利于颗粒在 GUV 膜上的吸附。之后，颗粒表面的疏水性进一步触发了颗粒进入 GUVs 的内化。这些结果表明了颗粒表面特性在其与脂质膜相互作用中的重要性，有助于理解细胞摄取机制。
