College of Plant Protection, Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Appl Microbiol Biotechnol. 2013 Aug;97(16):7131-9. doi: 10.1007/s00253-013-5013-8. Epub 2013 Jun 6.
1,2-Benzenedicarboxaldehyde-3,4,5-trihydroxy-6-methyl (flavipin) was found to be antagonistic against nematodes and fungi. Here we demonstrated that flavipin is a potent antioxidant in vitro and in vivo, which has great potential in the therapy for free radical-associated diseases. Therefore, flavipin-producing bio-source was screened from 80 endophytes in Ginkgo biloba. Seven endophytic fungi were able to synthesize antioxidant substances and identified by ITS rDNA sequences. Among them, Chaetomium globosum CDW7 was a remarkable producer of flavipin. The fermentation parameters of CDW7 were then optimized for high flavipin production. Cultured under the optimal condition (25 °C, 100/250 mL flask, 12 discs/flask, 150 rpm, pH 6.5) for 14 days, CDW7 was able to synthesize flavipin at a production of 315.5 mg/L. In addition, flavipin output was positively correlated to antioxidant activities of crude extracts with a correlation coefficient of 0.8235, indicating that flavipin was the major antioxidant component of CDW7's metabolites. These data demonstrated that CDW7 was a highly yielded bio-source of antioxidant flavipin.
1,2-苯二甲醛-3,4,5-三羟基-6-甲基（flavipin）被发现对线虫和真菌具有拮抗作用。在这里，我们证明 flavipin 是一种有效的抗氧化剂，无论是在体外还是体内，都具有治疗自由基相关疾病的巨大潜力。因此，我们从银杏的 80 种内生菌中筛选出了产生 flavipin 的生物源。通过 ITS rDNA 序列鉴定了 7 种能够合成抗氧化物质的内生真菌。其中，Chaetomium globosum CDW7 是 flavipin 的显著产生菌。然后对 CDW7 的发酵参数进行了优化，以提高 flavipin 的产量。在最佳条件（25°C、100/250 mL 摇瓶、12 片/瓶、150 rpm、pH 6.5）下培养 14 天，CDW7 能够合成 315.5 mg/L 的 flavipin。此外，flavipin 的产量与粗提物的抗氧化活性呈正相关，相关系数为 0.8235，表明 flavipin 是 CDW7 代谢物中主要的抗氧化成分。这些数据表明，CDW7 是一种高产抗氧化 flavipin 的生物源。
