Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mol Reprod Dev. 2013 Sep;80(9):718-24. doi: 10.1002/mrd.22202. Epub 2013 Jul 1.
Seminal proteins can be considered as factors that control fertilization. Clusterin is one such protein that has been implicated in many activities, including apoptosis inhibition, cell cycle control, DNA repair, and sperm maturation. In this study, the relationship between human secretory clusterin (sCLU) in seminal plasma with sperm parameters, protamine deficiency, and DNA fragmentation was investigated. Semen samples were collected from 63 Iranian men, and semen analysis was performed according to World Health Organization criteria and computer aided semen analysis (CASA). The concentration of sCLU in seminal plasma was measured by enzyme-linked immunosorbant assay (ELISA), protamine deficiency was determined by chromomycin A3 staining (CMA3 ), and sperm DNA fragmentation was checked by sperm chromatin dispersion (SCD) assay. The level of sCLU in seminal fluid of fertile patients was 48.3 ± 38.6 ng/ml and in infertile patients was 15.5 ± 9.7 ng/ml; this difference was significant (P < 0.001). sCLU correlated negatively with protamine deficiency, sperm DNA fragmentation, and abnormal morphology. In conclusion, seminal clusterin can be considered as a marker for the quick assessment of semen quality in male infertility studies.
精浆蛋白可以被视为控制受精的因素。 簇蛋白就是这样一种蛋白，它与许多活动有关，包括凋亡抑制、细胞周期控制、DNA 修复和精子成熟。 在这项研究中，研究了人分泌性簇蛋白（sCLU）与精液参数、鱼精蛋白缺乏和 DNA 碎片化之间的关系。 从 63 名伊朗男性中收集精液样本，并根据世界卫生组织标准和计算机辅助精液分析（CASA）进行精液分析。 通过酶联免疫吸附试验（ELISA）测量精浆中 sCLU 的浓度，通过吖啶橙染色（CMA3）测定鱼精蛋白缺乏，通过精子染色质扩散（SCD）试验检查精子 DNA 碎片化。 生育患者精浆中 sCLU 的水平为 48.3 ± 38.6 ng/ml，不育患者为 15.5 ± 9.7 ng/ml； 这种差异是显著的（P < 0.001）。 sCLU 与鱼精蛋白缺乏、精子 DNA 碎片化和形态异常呈负相关。 总之，精浆簇蛋白可以作为男性不育研究中精液质量快速评估的标志物。
