Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
Division of Enteric Bacterial Infections, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
J Med Microbiol. 2013 Sep;62(Pt 9):1338-1342. doi: 10.1099/jmm.0.059428-0. Epub 2013 Jun 5.
This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumoniae were identified from 277 Enterobacteriaceae clinical isolates. All were sequence type (ST) 258 and they had the same pulsotype. They had high MICs for carbapenems and multi-drug resistance. TEM-1, SHV-11 and OXA type β-lactamases were detected in all isolates, whereas CTX-M type β-lactamases and plasmid-mediated AmpC β-lactamase (PABL) were not present. A conjugation experiment failed, but blaKPC-2-harbouring plasmids from the six isolates were used to transform Escherichia coli DH5-α by electroporation. Each of the transformants harboured a blaKPC-2-positive approximately 95 kb plasmid, which was typed in the IncFII incompatibility group and co-harboured TEM-1 and OXA-9 β-lactamases. They shared the same restriction profile. This study confirms the emergence of clonal ST258 KPC-2-producing K. pneumoniae in some regions of Korea.
本研究分析了韩国近期产碳青霉烯酶肺炎克雷伯菌（KPC-2）的肺炎克雷伯菌分离株的特征和遗传相似性。最近的实验室监测发现，韩国产碳青霉烯酶肠杆菌科的数量有所增加。从 277 株肠杆菌科临床分离株中鉴定出 6 株产 KPC-2 的肺炎克雷伯菌。它们均为 ST258 型，脉冲场凝胶电泳图谱相同。它们对碳青霉烯类药物的 MIC 值较高，且具有多药耐药性。所有分离株均检测到 TEM-1、SHV-11 和 OXA 型β-内酰胺酶，而 CTX-M 型β-内酰胺酶和质粒介导的 AmpC β-内酰胺酶（PABL）不存在。虽然接合实验失败，但从 6 株分离株中提取的 blaKPC-2 携带质粒通过电穿孔转化为大肠杆菌 DH5-α。每个转化体均携带 blaKPC-2 阳性约 95 kb 的质粒，该质粒属于 IncFII 不相容群，同时携带 TEM-1 和 OXA-9 β-内酰胺酶。它们具有相同的限制酶图谱。本研究证实了克隆 ST258 型产 KPC-2 的肺炎克雷伯菌在韩国一些地区的出现。
