Thippeswamy B, Shivakumar C K, Krishnappa M
Dept. of Post Graduate Studies and Research in Microbiology, Kuvempu University, Shankaraghatta-577 451, India.
J Environ Biol. 2012 Nov;33(6):1063-8.
In the present study Aspergillus niger and Aspergillus flavus isolated from paper mill effluent showed tolerance and accumulation of toxic metals Ni, Zn, Cd, Pb, Cr and Cu from synthetic medium and paper mill effluent. Physico-chemical and heavy metals characterization of industrially treated paper mill effluent showed insignificant reduction in BOD, hardness, TDS and heavy metals as compared to permissible limits of BIS and WHO. A. niger and A. flavus were treated with synthetic medium containing 100-1000 mg l(-1) of six heavy metals. A. niger was able to tolerate and grow in 1000 mg l(-1) Pb, 500 mg l(-1) Cu, 250 mg l(-1) Zn and 100 mg l(-1) Cr, Ni respectively. No growth of A. niger was observed in 100 mg l-(-1) of Cd. A. flavus was capable to tolerate and grow in 1000 mg l(-1) Pb, Zn and Ni, 100mg l(-1) Cu. A. flavus growth was completely inhibited in 100 mg l(-1) of Cd and Cr. The Cd, Zn, Cu and Pb reduction were found significant (p < 0.05) in the paper effluent inoculated with A. niger and A. flavus biomass compared to industrial treated effluent. A. niger and A. flavus accumulated maximum of Pb (75.82%) followed by Zn (49.40%) > Cu (45.34%) > Ni (25.20%), while only 41% Cr was accumulated by A. nigerfrom 100 mg l(-1) of Cr solution.
在本研究中，从造纸厂废水中分离出的黑曲霉和黄曲霉表现出对合成培养基及造纸厂废水中有毒金属镍、锌、镉、铅、铬和铜的耐受性及积累能力。经工业处理的造纸厂废水的理化特性和重金属特征表明，与印度标准局（BIS）和世界卫生组织（WHO）的允许限值相比，其生化需氧量（BOD）、硬度、总溶解固体（TDS）和重金属的降低并不显著。用含有100 - 1000毫克/升六种重金属的合成培养基处理黑曲霉和黄曲霉。黑曲霉能够在1000毫克/升的铅、500毫克/升的铜、250毫克/升的锌和100毫克/升的铬、镍中耐受并生长。在100毫克/升的镉中未观察到黑曲霉生长。黄曲霉能够在1000毫克/升的铅、锌和镍、100毫克/升的铜中耐受并生长。在100毫克/升的镉和铬中，黄曲霉的生长完全受到抑制。与工业处理后的废水相比，接种黑曲霉和黄曲霉生物质的造纸厂废水中的镉、锌、铜和铅的减少量显著（p < 0.05）。黑曲霉和黄曲霉对铅的积累量最大（75.82%），其次是锌（49.40%）>铜（45.34%）>镍（25.20%），而黑曲霉从100毫克/升的铬溶液中仅积累了41%的铬。
