Gjelstad Astrid, Rasmussen Knut Einar, Parmer Marthe Petrine, Pedersen-Bjergaard Stig
School of Pharmacy, University of Oslo, PO Box 1068 Blindern, 0316 Oslo, Norway.
Bioanalysis. 2013 Jun;5(11):1377-85. doi: 10.4155/bio.13.59.
This paper reports development of a new approach towards analytical liquid-liquid-liquid membrane extraction termed parallel artificial liquid membrane extraction. A donor plate and acceptor plate create a sandwich, in which each sample (human plasma) and acceptor solution is separated by an artificial liquid membrane. Parallel artificial liquid membrane extraction is a modification of hollow-fiber liquid-phase microextraction, where the hollow fibers are replaced by flat membranes in a 96-well plate format.
Four basic drugs (pethidine, nortriptyline, methadone and haloperidol) were extracted from human plasma in 30 min, followed by analysis with LC-MS/MS. Extraction recoveries for the model analytes were in the range of 34-74% from human plasma. LOQs were in the range of 0.01-0.35 ng/ml, linearity above 0.9955 for all drugs and with RSD values below 12%.
Liquid-liquid-liquid membrane extraction was successfully performed in a slightly modified commercially available 96-well plate format.
本文报道了一种新的分析液-液-液膜萃取方法的开发，即平行人工液膜萃取。供体板和受体板形成一个夹层结构，其中每个样品（人血浆）和受体溶液被人工液膜隔开。平行人工液膜萃取是对中空纤维液相微萃取的一种改进，在96孔板形式中用平板膜代替了中空纤维。
在30分钟内从人血浆中萃取了四种碱性药物（哌替啶、去甲替林、美沙酮和氟哌啶醇），随后用LC-MS/MS进行分析。模型分析物从人血浆中的萃取回收率在34%-74%范围内。定量限在0.01-0.35 ng/ml范围内，所有药物的线性度均高于0.9955，相对标准偏差值低于12%。
液-液-液膜萃取在稍加改进的市售96孔板形式中成功进行。
