Liao W, Zeng F, Kang K, Qi Y, Yao L, Yang H, Ling L, Wu N, Wu D
Chongqing Ninth People's Hospital, Chongqing, China.
Transplant Proc. 2013 Jul-Aug;45(6):2451-4. doi: 10.1016/j.transproceed.2013.01.069. Epub 2013 Jun 3.
To study the role of lipoxin A4 (LXA4) in rat liver transplant rejection.
An acute rejection model of liver transplantation was established in inbred rats DA to LEW that were randomly divided into a control group and a LXA4 group. Liver morphologic changes were examined using hematoxylin/eosin staining. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified to measure liver injury. Intragraft mRNA and protein expressions of interferon (IFN)-γ interleukin and (IL)-10 were detected by real-time polymerase chain reaction and Western blots, respectively. The serum levels of IFN-γ and IL-10 were assayed by enzyme-linked immunosorbent assay.
LXA4 treatment improved hepatic tissue injury as indicated by morphologic analysis. Serum ALT and AST levels were significantly decreased at day 7 post-transplantation (P < .05). Concurrently, expression of IFN-γ was downregulated (P < .05) and secretion of IL-10 was enhanced (P < .05).
LXA4 attenuated acute rejection with a parallel shift from Th1 to Th2 responses in rat liver transplantation.
研究脂氧素A4（LXA4）在大鼠肝移植排斥反应中的作用。
在近交系大鼠DA至LEW中建立肝移植急性排斥模型，将其随机分为对照组和LXA4组。采用苏木精/伊红染色检查肝脏形态学变化。定量检测丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）以评估肝损伤。分别通过实时聚合酶链反应和蛋白质印迹法检测移植肝内干扰素（IFN）-γ、白细胞介素（IL）-10的mRNA和蛋白表达。采用酶联免疫吸附测定法检测血清中IFN-γ和IL-10水平。
形态学分析表明，LXA4治疗改善了肝组织损伤。移植后第7天血清ALT和AST水平显著降低（P <.05）。同时，IFN-γ表达下调（P <.05），IL-10分泌增强（P <.05）。
在大鼠肝移植中，LXA4减轻了急性排斥反应，同时伴随着从Th1反应向Th2反应的平行转变。
