Sadamoto Y, Arase S, Kato S, Nakanishi H, Fujie K, Takeda E, Takeda K
Department of Dermatology, School of Medicine, University of Tokushima.
Nihon Hifuka Gakkai Zasshi. 1990 Feb;100(2):211-4.
We examined the effects of 1, 25-dihydroxyvitamin D3 on the growth and differentiation of cultured human hair follicle cells from a patient with vitamin D-dependent rickets type II [DDR-II] with alopecia. In the presence of 100 nM 1, 25-dihydroxyvitamin D3, the clonal growth of normal hair follicle cells was inhibited and terminal differentiation was markedly stimulated. However, the cells from a patient with DDR-II with alopecia did not respond to the hormone, suggesting a lack of the specific receptors in the cells. The difference in the cellular response to the hormone between the normal hair follicle cells and those from a patient were clear and easily distinguished, and these studies may provide a basis for the rapid diagnosis of DDR-II. Large number of hairs were difficult to obtain from patients with alopecia, and we developed a new culture method to accomplish these studies. Our system may be useful in the culture of HFC from only a few hair follicles.
我们研究了1,25 - 二羟维生素D3对一名患有维生素D依赖性佝偻病II型（DDR-II）并伴有脱发的患者的培养人毛囊细胞生长和分化的影响。在100 nM 1,25 - 二羟维生素D3存在的情况下，正常毛囊细胞的克隆生长受到抑制，终末分化受到明显刺激。然而，患有DDR-II并伴有脱发的患者的细胞对该激素无反应，提示这些细胞中缺乏特异性受体。正常毛囊细胞与患者毛囊细胞对该激素的细胞反应差异明显且易于区分，这些研究可能为DDR-II的快速诊断提供依据。脱发患者难以获取大量毛发，我们开发了一种新的培养方法来完成这些研究。我们的系统可能有助于仅从少数毛囊培养毛囊细胞。
