Nagamori S
Department of Urology, Hokkaido University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1990 Apr;81(4):593-600. doi: 10.5980/jpnjurol1989.81.593.
DNA content in renal cell carcinoma was investigated to examine the tumor heterogeneity and was correlated with their morphologic grades. A total number of 147 paraffin-embedded samples (2-6 samples with a mean of 5) from 30 tumors were analyzed by flow cytometry. DNA aneuploid patterns were demonstrated in 5 of 9 grade 1 tumors (56%), 15 of 18 grade 2 tumors (83%) and 3 of all grade 3 tumors (100%), while aneuploid DNA histograms were exhibited in 20 of 57 grade 1 samples (35%), 45 of 81 grade 2 samples (56%) and 8 of 9 grade 3 samples (89%). DNA aneuploid patterns were demonstrated more significantly in grade 2 and 3 samples than in grade 1 samples (p less than 0.02 and p less than 0.01, respectively). Consequently, 73 samples (50%) showed DNA aneuploid patterns and 74 samples exhibited DNA diploid patterns. Eleven tumors (37%) showed homogeneous DNA ploidy patterns (7 tumors were diploid and 4 tumors were aneuploid only), while 19 tumors (63%) showed DNA heterogeneity, 17 of these 19 tumors demonstrating diploid as well as aneuploid samples. DNA heterogeneity was not found when the tumor samples examined were fewer than 3 samples. However, 19 of 25 tumors (76%) in which more than 4 samples were examined showed DNA heterogeneity, the incidence of which tended to be increased with upgrading of the tumor--3 of 9 (33%) in grade 1 tumors, 13 of 18 (72%) in grade 2 tumors and 3 of all (100%) in grade 3 tumors.(ABSTRACT TRUNCATED AT 250 WORDS)
对肾细胞癌的DNA含量进行了研究，以检查肿瘤的异质性，并将其与形态学分级相关联。通过流式细胞术分析了来自30个肿瘤的总共147个石蜡包埋样本（2 - 6个样本，平均为5个）。9个1级肿瘤中有5个（56%）显示出DNA非整倍体模式，18个2级肿瘤中有15个（83%），所有3级肿瘤中有3个（100%）；而在57个1级样本中有20个（35%）、81个2级样本中有45个（56%）、9个3级样本中有8个（89%）呈现非整倍体DNA直方图。2级和3级样本中DNA非整倍体模式的表现比1级样本更显著（分别为p < 0.02和p < 0.01）。因此，73个样本（50%）显示出DNA非整倍体模式，74个样本呈现DNA二倍体模式。11个肿瘤（37%）显示出均匀的DNA倍性模式（7个肿瘤为二倍体，4个肿瘤仅为非整倍体），而19个肿瘤（63%）显示出DNA异质性，这19个肿瘤中有17个同时显示出二倍体和非整倍体样本。当检查的肿瘤样本少于3个时未发现DNA异质性。然而，在检查了超过4个样本的25个肿瘤中有19个（76%）显示出DNA异质性，其发生率倾向于随着肿瘤分级的升高而增加——1级肿瘤中9个中有3个（33%），2级肿瘤中18个中有13个（72%），3级肿瘤中所有3个（100%）。（摘要截短于250字）
