Logan B K, Stafford D T, Tebbett I R, Moore C M
Department of Pathology, University of Tennessee, Memphis 38163.
J Anal Toxicol. 1990 May-Jun;14(3):154-9. doi: 10.1093/jat/14.3.154.
A system involving the combination of cation exchange solid-phase extraction and gradient elution HPLC with diode array detection was developed for the isolation and identification of basic drugs in urine. At least 100 basic drugs commonly encountered in urinary drug screening and many popular illicit drugs could be identified. Detailed study of 30 of these compounds showed that in addition to the procedure's simplicity and convenience, recoveries were close to 100% in most cases. The method was compared with an existing liquid-liquid extraction/capillary gas chromatography procedure and was at least as effective for extracting and identifying basic drugs, and especially effective at extracting problematic compounds including morphine, benzoylecgonine, temazepam, and oxazepam. Additional advantages of solid-phase extraction technology include the elimination of time-consuming shaking and centrifugation steps, elimination of reusable glassware, and reduced sample-operator contact with potentially infectious samples.
开发了一种将阳离子交换固相萃取与梯度洗脱高效液相色谱及二极管阵列检测相结合的系统，用于尿液中碱性药物的分离与鉴定。至少可鉴定出尿液药物筛查中常见的100种碱性药物以及许多常见的非法药物。对其中30种化合物的详细研究表明，该方法除了简单方便外，大多数情况下回收率接近100%。将该方法与现有的液 - 液萃取/毛细管气相色谱法进行比较，结果表明其在提取和鉴定碱性药物方面至少同样有效，尤其在提取包括吗啡、苯甲酰爱康宁、替马西泮和奥沙西泮等疑难化合物方面效果显著。固相萃取技术的其他优点包括省去了耗时的振荡和离心步骤，无需使用可重复使用的玻璃器皿，以及减少了样品操作人员与潜在传染性样品的接触。
