Ruangyuttikarn W, Law M Y, Rollins D E, Moody D E
Department of Pharmacology and Toxicology, College of Pharmacy, University of Utah, Salt Lake City 84112.
J Anal Toxicol. 1990 May-Jun;14(3):160-4. doi: 10.1093/jat/14.3.160.
A prototype enzyme-linked immunosorbent assay (ELISA) for fentanyl, a potent synthetic narcotic analgesic, was used to detect the presence of fentanyl and its analogs in human urine. Human drug-free urine was spiked with 0.02 to 100 ng/mL of fentanyl or with 0.1 to 500 ng/mL of each analog and used as a sample in the ELISA. Sensitivity and precision of the assay were analyzed specifically for detection of fentanyl. Cross-reactivities of fentanyl and nine analogs, acetylfentanyl, p-methylfentanyl, p-fluorofentanyl, butyrylfentanyl, (+/-)trans-3-methylfentanyl, (+/-)cis-3-methylfentanyl, alpha-methylfentanyl, benzylfentanyl, and alfentanil for this assay were also determined. The results indicate that the ELISA was capable of detecting urinary fentanyl with a limit of detection of 0.25 ng/mL and a reasonable "working cutoff" of 0.5 ng/mL. The assay was moderately precise with within-run precision, determined at fentanyl concentrations of 10.0 and 2.5 ng/mL, showing coefficients of variation from 7.7 to 10.4% and between-run precision, determined at concentrations of 10.0 and 2.5 ng/mL, showing coefficients of variation from 14.2 to 23.7%. Acetylfentanyl, p-fluorofentanyl, p-methylfentanyl, and butyrylfentanyl show good cross-reactivity in the assay relative to fentanyl. The cross-reactivities equivalent to 2 ng/mL of fentanyl for acetylfentanyl, p-fluorofentanyl, p-methylfentanyl, and butyrylfentanyl were 111, 93, 87, and 77%, respectively. The (+/-)trans-3-methylfentanyl, alpha-methylfentanyl, and (+/-)cis-3-methylfentanyl show less cross-reactivity; their percent cross-reactivities equivalent to 2 ng/mL of fentanyl were 50, 19, and 3%, respectively. Benzylfentanyl and alfentanil, however, do not show any cross-reactivity.(ABSTRACT TRUNCATED AT 250 WORDS)
一种用于检测强效合成麻醉性镇痛药芬太尼的酶联免疫吸附测定（ELISA）原型，被用于检测人尿液中芬太尼及其类似物的存在。将不含药物的人尿液分别加入浓度为0.02至100 ng/mL的芬太尼或浓度为0.1至500 ng/mL的每种类似物，用作ELISA检测的样本。专门分析了该检测方法对芬太尼检测的灵敏度和精密度。还测定了芬太尼及其九种类似物（乙酰芬太尼、对甲基芬太尼、对氟芬太尼、丁酰芬太尼、（±）反式-3-甲基芬太尼、（±）顺式-3-甲基芬太尼、α-甲基芬太尼、苄基芬太尼和阿芬太尼）在此检测方法中的交叉反应性。结果表明，该ELISA能够检测尿液中的芬太尼，检测限为0.25 ng/mL，合理的“工作截断值”为0.5 ng/mL。该检测方法的精密度适中，在芬太尼浓度为10.0和2.5 ng/mL时测定的批内精密度，变异系数为7.7%至10.4%，在浓度为10.0和2.5 ng/mL时测定的批间精密度，变异系数为14.2%至23.7%。乙酰芬太尼、对氟芬太尼、对甲基芬太尼和丁酰芬太尼在该检测方法中相对于芬太尼表现出良好的交叉反应性。乙酰芬太尼、对氟芬太尼、对甲基芬太尼和丁酰芬太尼相当于2 ng/mL芬太尼的交叉反应率分别为111%、93%、87%和77%。（±）反式-3-甲基芬太尼、α-甲基芬太尼和（±）顺式-3-甲基芬太尼的交叉反应性较低；它们相当于2 ng/mL芬太尼的交叉反应率分别为50%、19%和3%。然而，苄基芬太尼和阿芬太尼未表现出任何交叉反应性。（摘要截断于250字）
