[ABO血型鉴定不符病例的分子基础与临床输血]
[Molecular basis and clinical blood transfusion for cases with ABO typing discrepancy].
作者信息
Zhang Zhe, Deng Gang, Huang Dandan, Guo Wenyu, Xu Deyi, Du Yong, Tang Xiaoxian, Yu Yong, Deng Danfei
机构信息
Ningbo Central Blood Station, Ningbo, Zhejiang 315040, P.R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2013 Jun;30(3):326-9. doi: 10.3760/cma.j.issn.1003-9406.2013.03.017.
OBJECTIVE
To investigate the molecular genetic basis of samples with ABO typing discrepancy and provide the guidline for identification and clinical transfusion for these samples.
METHODS
Six cases with similar serological characteristics were collected. Serological method, PCR-SSP and direct sequencing of ABO gene were used to explore the underlying mechanism. Condition of clinical transfusion of patients was also reviewed.
RESULTS
Three conditions were related with the ABO blood type discrepancy, which included weaken antigen (2 cases), weakened antibody (3 cases) and ABO subtype (1 case). The satisfactory effect of transfusion was achieved in all patients with the principle of the same blood type or the compatible crossmatch.
CONCLUSION
Heterogeneity has existed with the ABO group. Indivianals with same reaction pattern may result in different mechanisms.
目的
探讨ABO血型定型不符样本的分子遗传学基础，为这些样本的鉴定及临床输血提供指导。
方法
收集6例血清学特征相似的病例。采用血清学方法、聚合酶链反应-序列特异性引物（PCR-SSP）及ABO基因直接测序法探究其潜在机制。同时回顾患者的临床输血情况。
结果
3种情况与ABO血型不符有关，包括抗原减弱（2例）、抗体减弱（3例）及ABO亚型（1例）。所有患者均按照同型输血或交叉配血相合的原则输血，效果满意。
结论
ABO血型存在异质性。反应模式相同的个体可能存在不同的机制。
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