The State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, 100005, People's Republic of China.
Mol Cell Biochem. 2013 Oct;382(1-2):127-36. doi: 10.1007/s11010-013-1726-3. Epub 2013 Jun 7.
In China, the traditional Chinese medicine "YiSui ShenXu Granule" has been used for treating β-thalassemia over 20 years and known to be effective in clinic. Several purified components from "YiSui ShenXu Granule" are tested in K562 cells to reveal its effect on globin expression and erythroid differentiation, and one of the purified components, emodin, was demonstrated to increase the expression of α-, ε-, γ-globin, CD235a, and CD71 in K562 cells. Moreover, the increase of their expression is emodin concentration-dependent. The mRNA and microRNA (miRNA) expression profiles are further analyzed and 417 mRNAs and 35 miRNAs with differential expression between untreated and emodin-treated K562 cells were identified. Among them, two mRNAs that encode known positive regulators of erythropoiesis, ALAS2, and c-KIT respectively, increased during emodin-induced K562 erythroid differentiation, meanwhile, two negative regulators, miR-221 and miR-222, decreased during this process. These results indicate that emodin can improve the expression of globin genes in K562 cells and also induce K562 cells to erythroid differentiation possibly through up-regulating ALAS2 and c-KIT and down-regulating miR-221 and miR-222.
在中国，中药“益髓生血颗粒”已被用于治疗β-地中海贫血超过 20 年，临床疗效确切。从“益髓生血颗粒”中提取的几种纯化成分在 K562 细胞中进行测试，以揭示其对珠蛋白表达和红细胞分化的影响，其中一种纯化成分大黄素被证明能增加 K562 细胞中α、ε、γ-珠蛋白、CD235a 和 CD71 的表达。此外，这种增加是大黄素浓度依赖性的。进一步分析 mRNA 和 microRNA (miRNA) 表达谱，在未处理和大黄素处理的 K562 细胞之间鉴定出 417 个差异表达的 mRNA 和 35 个 miRNA。其中，编码已知的红细胞生成正调控因子的两种 mRNA，ALAS2 和 c-KIT，在大黄素诱导的 K562 红细胞分化过程中增加，同时，两种负调控因子，miR-221 和 miR-222，在此过程中减少。这些结果表明，大黄素可以改善 K562 细胞中珠蛋白基因的表达，并通过上调 ALAS2 和 c-KIT 以及下调 miR-221 和 miR-222 诱导 K562 细胞向红细胞分化。
