Jackson H, Rabellino E, Williams N
Department of Physiology, Melbourne University, Victoria, Australia.
Leukemia. 1990 Jul;4(7):490-3.
Human megakaryocyte development was studied in cell culture by first determining the differentiation stage of the clonable progenitor cell population and then analyzing the kinetics of expression of two megakaryocyte antigens using an immunoalkaline phosphatase system. Studies on the expression of two antigens present on megakaryocytes but not detectable on platelets (Mk-A, Mk-B) revealed that Mk-A antigen was found on megakaryocytes in colonies of less than six to eight cells at early times of cell culture. By contrast, Mk-B antigen was detected only in large megakaryocytes starting day 7 of culture and observed primarily in colonies of greater than eight cells. These studies show that human megakaryocyte progenitors are among the more mature of the precursor classes and link the antigen A bearing megakaryocytes to the developmental sequence as the immediate progeny of the precursor population with limited mitotic capacity. Amplification of antigen B expression is late in the developmental process, being detected by this method on only large megakaryocytes in well-formed colonies after 7-9 days of culture.
通过首先确定可克隆祖细胞群体的分化阶段，然后使用免疫碱性磷酸酶系统分析两种巨核细胞抗原的表达动力学，在细胞培养中研究了人类巨核细胞的发育。对巨核细胞上存在但在血小板上无法检测到的两种抗原（Mk-A、Mk-B）的表达研究表明，在细胞培养早期，Mk-A抗原在少于六到八个细胞的集落中的巨核细胞上被发现。相比之下，Mk-B抗原仅在培养第7天开始的大型巨核细胞中被检测到，并且主要在大于八个细胞的集落中观察到。这些研究表明，人类巨核细胞祖细胞是较成熟的前体细胞类别之一，并将携带抗原A的巨核细胞与发育序列联系起来，作为具有有限有丝分裂能力的前体细胞群体的直接后代。抗原B表达的放大在发育过程中较晚，通过这种方法在培养7-9天后仅在形态良好的集落中的大型巨核细胞上检测到。
