Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Institute of Biological Engineering, Donghua University, Shanghai, 201620, China.
J Biomed Mater Res B Appl Biomater. 2013 Jun 7:471-478. doi: 10.1002/jbm.b.32972.
Electrospinning is one of the most simple and effective methods to prepare polymer fibers with the diameters ranging from nanometer to several micrometers. Poly(L-lactide)-co-poly (ɛ-caprolactone) (P(LLA-CL)) fibers and P(LLA-CL)/heparin coaxial composite fibers herein were successfully prepared by single electrospinning and coaxial electrospinning, respectively. The prepared endothelialized P(LLA-CL) and P(LLA-CL)/heparin vascular grafts were used in the Beagle dogs experiment to evaluate the feasibility of thus made different scaffolds for substitution of dog femoral artery in early period, medium term, and long term, meanwhile the pure P(LLA-CL) vascular graft was used as the control group during all the experiments. The animal model was established by using the graft materials to anastomose both femoral arteries of dogs. The vascular grafts patency rates (i.e., the unobstructed capacity of blood vessel) were detected by color Doppler flow imaging technology and digital subtraction angiography. To observe the histological morphology at different periods, the vascular grafts were removed after 7, 14, and 30 days, and the corresponding histological changes were evaluated by hematoxylin and eosin staining. The experimental results show that in the early period, the patency rates of pure P(LLA-CL) graft, endothelial P(LLA-CL) graft, and P(LLA-CL)/heparin graft were 75%, 75%, and 100%, respectively; in the medium term, the patency rates of pure P(LLA-CL) graft and endothelial P(LLA-CL) graft were 25%, whereas that of P(LLA-CL)/heparin graft was 50%; the patency rates of pure P(LLA-CL) graft and endothelial P(LLA-CL) graft were down to 0%, whereas the patency rate of P(LLA-CL)/heparin graft was 25% in the long term. This preliminary study has demonstrated that P(LLA-CL)/heparin coaxial composite fiber maybe a reliable artificial graft for the replacement of femoral artery. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
静电纺丝是一种最简单、最有效的方法，可制备直径从纳米到几微米的聚合物纤维。本文分别通过单静电纺丝和同轴静电纺丝成功制备了聚（L-丙交酯）-共-聚（ε-己内酯）（P（LLA-CL））纤维和 P（LLA-CL）/肝素同轴复合纤维。将制备的内皮化 P（LLA-CL）和 P（LLA-CL）/肝素血管移植物用于比格犬实验，以评估不同支架在早期、中期和晚期替代狗股动脉的可行性，同时在所有实验中，纯 P（LLA-CL）血管移植物作为对照组。通过使用移植物材料吻合狗的两条股动脉建立动物模型。通过彩色多普勒血流成像技术和数字减影血管造影检测血管移植物的通畅率（即血管通畅能力）。为了观察不同时期的组织形态学变化，在第 7、14 和 30 天分别取出血管移植物，并通过苏木精和伊红染色评估相应的组织学变化。实验结果表明，在早期，纯 P（LLA-CL）移植物、内皮化 P（LLA-CL）移植物和 P（LLA-CL）/肝素移植物的通畅率分别为 75%、75%和 100%；在中期，纯 P（LLA-CL）移植物和内皮化 P（LLA-CL）移植物的通畅率为 25%，而 P（LLA-CL）/肝素移植物的通畅率为 50%；在晚期，纯 P（LLA-CL）移植物和内皮化 P（LLA-CL）移植物的通畅率降至 0%，而 P（LLA-CL）/肝素移植物的通畅率为 25%。这项初步研究表明，P（LLA-CL）/肝素同轴复合纤维可能是一种可靠的股动脉替代人工移植物。© 2013 Wiley Periodicals, Inc. J 生物材料 Res 部分 B: 应用生物材料, 2013。
