Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.
Chemistry. 2013 Jul 15;19(29):9511-7. doi: 10.1002/chem.201301273. Epub 2013 Jun 6.
A series of five europium(III) complexes has been prepared from heptadentate N5O2 ligands that possess a brightness of more than 10 mM(-1) cm(-1) in water, following excitation over the range λ=330-355 nm. Binding of several oxy anions has been assessed by emission spectral titrimetric analysis, with the binding of simple carboxylates, lactate and citrate involving a common ligation mode following displacement of the coordinated water. Selectivity for bicarbonate allows the rapid determination of this anion in human serum, with K(d)=37 mM (295 K). The complexes are internalised quickly into mammalian cells and exhibit a mitochondrial localisation at early time points, migrating after a few hours to reveal a predominant lysosomal distribution. Herein, we report the synthesis and complexation behaviour of strongly emissive europium (III) complexes that bind oxy-anions in aqueous media with an affinity and selectivity profile that is distinctively different from previously studied systems.
已制备了一系列具有七齿 N5O2 配体的五铕（III）配合物，在 λ=330-355nm 范围内激发后，其在水中的亮度超过 10mM(-1)cm(-1)。通过发射光谱滴定分析评估了几种含氧阴离子的结合情况，简单的羧酸根、乳酸盐和柠檬酸盐的结合涉及到配位水置换后的常见配位模式。对碳酸氢盐的选择性允许快速测定人血清中的这种阴离子，K(d)=37mM(295K)。这些配合物很快被内吞到哺乳动物细胞中，并在早期表现出线粒体定位，几个小时后迁移，显示出主要的溶酶体分布。在此，我们报告了强发光铕（III）配合物的合成和配位行为，该配合物在水相中与含氧阴离子结合，其亲和力和选择性与以前研究的系统明显不同。
