Mal'tseva O V, Golovleva L A
Mikrobiologiia. 1990 Jan-Feb;59(1):163-5.
Peripheral metabolism was studied in the Pseudomonas putida 37cc transconjugant. In the strain grown on benzoate, pyrocatechase (PC) I with a low activity to chlorocatechols was induced, whereas PCII actively decomposing chlorocatechols was induced during its growth on 3-chlorobenzoic acid. The P. putida 37cc transconjugant grown on alpha-methylstyrene (MS) exerted the activity of both metapyrocatechase (MPC) and PC, whereas in the parent strain P. putida R-1 only MPC was involved in the degradation of alpha-MS. The substrate specificity of the enzymes involved in the ring cleavage by P. putida 37cc was compared to show that, apparently, MPC of the transconjugant was similar to this enzyme in the strain R-1 while PC decomposing chlorocatechols was similar to PC of the P. putida 87 donor. The regulation of the enzymes mediating the ring cleavage was studied in the parent strains and transconjugants.
对恶臭假单胞菌37cc转接合子的外周代谢进行了研究。在以苯甲酸盐为生长底物的菌株中，诱导产生了对氯儿茶酚活性较低的邻苯二酚酶（PC）I，而在以3-氯苯甲酸为生长底物时，诱导产生了能有效分解氯儿茶酚的PCII。在以α-甲基苯乙烯（MS）为生长底物的恶臭假单胞菌37cc转接合子中，间位邻苯二酚酶（MPC）和PC均有活性，而在亲本菌株恶臭假单胞菌R-1中，只有MPC参与α-MS的降解。比较了恶臭假单胞菌37cc中参与环裂解的酶的底物特异性，结果表明，转接合子的MPC显然与菌株R-1中的该酶相似，而分解氯儿茶酚的PC与恶臭假单胞菌87供体的PC相似。对亲本菌株和转接合子中介导环裂解的酶的调控进行了研究。
