Department of Biomedical Engineering, University of California, 451 Health Sciences Drive, #2519, Davis, CA 95616, USA.
Analyst. 2013 Aug 7;138(15):4321-6. doi: 10.1039/c3an00818e. Epub 2013 Jun 7.
Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α) - a key inflammatory cytokine - in whole human blood. When testing spiked blood, a TNF-α detection limit of 58 pM (10 ng mL(-1)) and a linear range of 6 nM (100 ng mL(-1)) could be achieved. Furthermore, detection of TNF-α in mitogen stimulated whole blood was demonstrated.
电化学适体传感器可用于检测疾病标志物，如细胞因子，从而提供低成本、快速、特异和敏感的即时诊断。在此，我们介绍了一种基于适体的电化学传感器的开发，用于检测和分析全血中的肿瘤坏死因子-α（TNF-α）——一种关键的炎症细胞因子。在检测加标血样时，该传感器对 TNF-α 的检测限为 58 pM（10 ng mL（-1）），线性范围为 6 nM（100 ng mL（-1））。此外，还证明了该传感器可用于检测有丝分裂原刺激的全血中的 TNF-α。
