Department of Diarrheal Diseases, National Institute for Communicable Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
Foodborne Pathog Dis. 2013 Aug;10(8):723-30. doi: 10.1089/fpd.2012.1441. Epub 2013 Jun 8.
Rapid and easy-to-use molecular subtyping methods are being explored and used for the surveillance of bacterial diseases, including multiple-loci variable number of tandem repeats (VNTR) analysis (MLVA). In this study, we assessed different VNTR combinations for the subtyping of Vibrio cholerae serogroups O1 and O139 with strain panels selected from a long-term nationwide cholera survey. By only using three highly variable loci (VC0147, VCA0171, and VCA0283), we acquired a high discriminatory power, which equals that found after using a combination of all nine loci and that of a pulsed-field gel electrophoresis analysis. Evaluation using the outbreak strains showed a good clustering of the three-loci MLVA (VC0147, VCA0171, and VCA0283). In addition, a six-loci MLVA (VC0147, VC0437, VC1457, VC1650, VCA0171, and VCA0283) protocol allowed for the clustering of O1/O139 V. cholerae strains, which have different serogroups/biotypes and toxigenic/nontoxigenic characteristics. Here, we propose that the three-loci MLVA can be utilized as a molecular subtyping protocol in cholera epidemiological investigations, and the six-loci MLVA can be used in phylogenetic and population structure analyses of V. cholerae O1/O139.
正在探索和使用快速且易于使用的分子亚型方法来监测细菌病，包括多位点可变数串联重复序列（VNTR）分析（MLVA）。在这项研究中，我们评估了不同的 VNTR 组合，用于对来自长期全国性霍乱调查的菌株进行 O1 和 O139 群霍乱弧菌的分型。仅使用三个高度可变的基因座（VC0147、VCA0171 和 VCA0283），我们获得了高分辨力，与使用所有九个基因座的组合和脉冲场凝胶电泳分析的分辨力相等。使用暴发菌株进行的评估表明，三基因座 MLVA（VC0147、VCA0171 和 VCA0283）的聚类良好。此外，六基因座 MLVA（VC0147、VC0437、VC1457、VC1650、VCA0171 和 VCA0283）方案允许对具有不同血清型/生物型和产毒性/非产毒性特征的 O1/O139 霍乱弧菌菌株进行聚类。在这里，我们提出三基因座 MLVA 可用于霍乱流行病学研究中的分子分型方案，六基因座 MLVA 可用于霍乱弧菌 O1/O139 的系统发育和种群结构分析。
