Sarroukh Rabia, Goormaghtigh Erik, Ruysschaert Jean-Marie, Raussens Vincent
Center for Structural Biology and Bioinformatics, Laboratory of Structure and Function of Biological Membrane, Faculté des Sciences, Université Libre de Bruxelles ULB, Campus de la Plaine CP 206/2, Boulevard du Triomphe, B-1050, Brussels, Belgium.
Biochim Biophys Acta. 2013 Oct;1828(10):2328-38. doi: 10.1016/j.bbamem.2013.04.012. Epub 2013 Jun 5.
Amyloid refers to insoluble protein aggregates that are responsible for amyloid diseases but are also implicated in important physiological functions (functional amyloids). The widespread presence of protein aggregates but also, in most of the cases, their deleterious effects explain worldwide efforts made to understand their formation, structure and biological functions. We emphasized the role of FTIR and especially ATR-FTIR techniques in amyloid protein and/or peptide studies. The multiple advantages provided by ATR-FTIR allow an almost continuous structural view of protein/peptide conversion during the aggregation process. Moreover, it is now well-established that infrared can differentiate oligomers from fibrils simply on their spectral features. ATR-FTIR is certainly the fastest and easiest method to obtain this information. ATR-FTIR occupies a key position in the analysis and comprehension of the complex aggregation mechanism(s) at the oligomer and/or fibril level. These mechanism(s) seem to present strong similarities between different amyloid proteins and might therefore be extremely important to understand for both disease-associated and functional amyloid proteins. This article is part of a Special Issue entitled: FTIR in membrane proteins and peptide studies.
淀粉样蛋白是指不溶性蛋白质聚集体，它们是导致淀粉样疾病的原因，但也与重要的生理功能（功能性淀粉样蛋白）有关。蛋白质聚集体广泛存在，而且在大多数情况下，它们具有有害作用，这解释了全球范围内为了解其形成、结构和生物学功能所做的努力。我们强调了傅里叶变换红外光谱（FTIR），尤其是衰减全反射傅里叶变换红外光谱（ATR-FTIR）技术在淀粉样蛋白和/或肽研究中的作用。ATR-FTIR提供的多种优势使得在聚集过程中能够几乎连续地观察蛋白质/肽的结构转变。此外，现在已经明确红外光可以仅根据光谱特征区分寡聚体和原纤维。ATR-FTIR无疑是获取此类信息最快且最简单的方法。ATR-FTIR在分析和理解寡聚体和/或原纤维水平上复杂的聚集机制方面占据关键地位。这些机制在不同的淀粉样蛋白之间似乎具有很强的相似性，因此对于理解与疾病相关的淀粉样蛋白和功能性淀粉样蛋白而言可能都极为重要。本文是名为“膜蛋白和肽研究中的FTIR”特刊的一部分。
