Department of Pharmacology, Ningxia Medical University, Yinchuan, China.
Phytomedicine. 2013 Aug 15;20(11):1039-45. doi: 10.1016/j.phymed.2013.04.012. Epub 2013 Jun 6.
In this study we investigated antinociceptive effects of oxymatrine through regulation of NR2B-containing NMDA receptor-ERK/CREB signaling in a chronic neuropathic pain model induced by chronic constrictive injury (CCI) of the sciatic nerve.
The von Frey and plantar tests were performed to assess the degree of mechanical and thermal changes respectively. Immunohistochemistry assay was used to evaluate the expressions of NR2B. Western blotting assay were used to evaluate the expressions of NR2B, tERK, p-ERK, tCREB and p-CREB.
The intraperitoneal administration of OMT (160, 80 mg/kg) could prevent the development of mechanical allodynia, thermal hyperalgesia induced by CCI. Intraperitoneal administration of OMT decreased the mean IOD of NR2B in the dorsal horn and expression of NR2B, p-ERK and p-CREB protein.
Regulation of NMDA NR2B receptor-ERK/CREB signaling maybe the targets for the antinociceptive effects of OMT on a chronic neuropathic pain model induced by chronic constrictive injury of the sciatic nerve.
本研究通过调控 NMDA 受体 NR2B-ERK/CREB 信号通路，探讨氧化苦参碱（OMT）对慢性坐骨神经缩窄性损伤（CCI）诱导的慢性神经病理性疼痛模型的镇痛作用。
采用von Frey 细丝和足底缩足反射试验分别评估机械性和热痛觉变化程度。免疫组织化学法检测 NR2B 的表达。Western blot 法检测 NR2B、tERK、p-ERK、tCREB 和 p-CREB 的表达。
腹腔给予 OMT（160、80mg/kg）可预防 CCI 诱导的机械性痛觉过敏和热痛觉超敏。腹腔给予 OMT 可降低背角 NR2B 的平均光密度和 NR2B、p-ERK 和 p-CREB 蛋白的表达。
调控 NMDA 受体 NR2B-ERK/CREB 信号通路可能是 OMT 对慢性坐骨神经缩窄性损伤诱导的慢性神经病理性疼痛模型产生镇痛作用的靶点。
