Kerckhoff Heart and Thorax Center, Department of Cardiology, Bad Nauheim, Germany.
J Am Coll Cardiol. 2013 Sep 10;62(11):992-8. doi: 10.1016/j.jacc.2013.05.025. Epub 2013 Jun 7.
This study sought to evaluate exact release kinetics of microRNAs (miRNAs) in acute myocardial infarction (AMI).
miRNAs may be useful as novel biomarkers in patients with cardiovascular disease, although it is difficult to establish the detailed release kinetics of miRNAs in patients with AMI.
We analyzed the release kinetics of circulating cardiac-specific (miR-21, miR-208a) and muscle-enriched (miR-1, miR-133a) miRNAs using the TaqMan polymerase chain reaction in patients with hypertrophic obstructive cardiomyopathy who were undergoing transcoronary ablation of septal hypertrophy (TASH), a procedure mimicking AMI. Consecutive patients (n = 21) undergoing TASH were included. Serum samples were collected prior to and at 15, 30, 45, 60, 75, 90, and 105 min and 2, 4, 8, and 24 h after TASH.
Circulating concentrations of miR-1 were significantly increased (>3-fold; p = 0.01) after 15 min, with a peak after 75 min (>60-fold; p < 0.001). The miR-21 concentrations were not increased at any time point. Concentrations of miR-133a were significantly increased at 15 min (2.9-fold; p < 0.001) and reached a plateau between 75 and 480 min (>50-fold change). The miR-208a concentrations were elevated at 105 min (>2-fold; p = 0.01), without a further increase.
miR-1, miR-133a, and miR-208a were continuously increased during the first 4 h after the induction of MI. In particular, miR-1 and miR-133a were significantly increased at early time points. These results demonstrate the release kinetics of miRNAs, which are helpful for developing their potential use as biomarkers in patients with acute coronary syndromes.
本研究旨在评估急性心肌梗死（AMI）中 microRNAs（miRNAs）的确切释放动力学。
miRNAs 可能作为心血管疾病患者的新型生物标志物具有一定价值，然而，难以确定 AMI 患者中 miRNAs 的详细释放动力学。
我们采用 TaqMan 聚合酶链反应分析了肥厚型梗阻性心肌病患者行经冠状消融室间隔肥厚术（TASH）过程中的循环心脏特异性（miR-21、miR-208a）和肌肉丰富型（miR-1、miR-133a）miRNAs 的释放动力学，该过程模拟 AMI。连续纳入 21 例行 TASH 的患者。在 TASH 前以及 TASH 后 15、30、45、60、75、90 和 105 分钟以及 2、4、8 和 24 小时采集血清样本。
miR-1 的循环浓度在 15 分钟后显著增加（>3 倍；p = 0.01），75 分钟后达到峰值（>60 倍；p < 0.001）。miR-21 的浓度在任何时间点均未增加。miR-133a 的浓度在 15 分钟时显著增加（2.9 倍；p < 0.001），并在 75 至 480 分钟之间达到平台期（>50 倍变化）。miR-208a 的浓度在 105 分钟时升高（>2 倍；p = 0.01），之后没有进一步增加。
在诱导 MI 后 4 小时内，miR-1、miR-133a 和 miR-208a 持续增加。特别是，miR-1 和 miR-133a 在早期时间点显著增加。这些结果证明了 miRNAs 的释放动力学，这有助于开发它们作为急性冠状动脉综合征患者生物标志物的潜在用途。
